Correlation between biofilm formation and production of polysaccharide intercellular adhesin in clinical isolates of coagulase-negative staphylococci
Correlation between biofilm formation and production of polysaccharide intercellular adhesin in clinical isolates of coagulase-negative staphylococci
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DOI：
10.1016/j.ijmm.2006.02.018
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发表时间：
2006-10-01
影响因子：
4.1
通讯作者：
Jabbouri, Said
中科院分区：
文献类型：
--
作者：
Chokr, Ali;Watier, Denis;Jabbouri, Said
形成生物膜的能力似乎在凝固酶阴性葡萄球菌（CoNS）的毒力中发挥重要作用，使其能够引起持续的人工器械相关感染。葡萄球菌生物膜的最明确表征的组分是由icaADBC操纵子编码的多糖细胞间粘附素（PIA）。在本文中，我们评估了形成生物膜（Bf+/-）、合成PIA（PIA+/-）的能力与伊卡位点（伊卡+/-）的存在之间的联系。为此，在体外测试了66株CoNS菌株。73%的菌株显示存在伊卡位点（伊卡+），因此可能能够产生PIA并形成生物膜。然而，观察到的特征表明，所有菌株中有15%形成生物膜而不产生PIA（Bf+，PIA-，伊卡+/-），而8%是PIA生产者而不形成生物膜（Bf-，PIA+，伊卡+）。基于所获得的数据，我们得出结论：（i）单独的PIA合成不足以产生生物膜，并且（ii）葡萄球菌也可以在不产生PIA的情况下形成生物膜。(c)2006年Elsevier GmbH。All rights reserved.
The ability to form a biofilm seems to play an essential role in the virulence of coagulase-negative staphylococci (CoNS) by permitting them to cause persistent prosthetic device-related infections. The most clearly characterized component of staphylococcal biofilms is the polysaccharide intercellular adhesin (PIA) encoded by the icaADBC operon. In the present paper, we assess the link between the ability to form a biofilm (Bf+/-), to synthesize PIA (PIA+/-) and the presence of the ica locus (ica+/-). For this purpose, 66 CoNS strains were tested in vitro. Seventy three percent of all strains revealed presence of the ica locus (ica+), and therefore were potentially able to produce PIA and to form a biofilm. However, the characteristics observed indicated that 15% of all strains were biofilm forming without PIA production (Bf+, PIA-, ica+/-) while 8% were PIA producers without biofilm formation (Bf-, PIA+, ica+). On the basis of the obtained data we conclude that (i) PIA synthesis alone is not sufficient to produce a biofilm and (ii) staphylococci can also form a biofilm without producing PIA. (c) 2006 Elsevier GmbH. All rights reserved.