Cellular behavior in the developing Drosophila pupal retina
Cellular behavior in the developing Drosophila pupal retina
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DOI：
10.1016/j.mod.2007.11.007
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发表时间：
2008-03-01
影响因子：
2.6
通讯作者：
Cagan, Ross L.
中科院分区：
文献类型：
--
作者：
Larson, David E.;Liberman, Zoe;Cagan, Ross L.
上皮内细胞的正确模式是建立其正常功能的关键。然而，单个细胞到达其最终发育生态位的精确机制仍然知之甚少。我们开发了一种优化的系统，用于对发育中的果蝇视网膜进行成像，这是研究细胞定位的理想组织。使用这种技术，我们表征了野生型蛹视网膜发育的细胞动力学。我们还分析了影响眼睛图案的两种突变体，并证明Notch或Roughest信号传导的细胞突变体的细胞运动异常动态。最后，我们通过调节正常粘附连接完整性，确定了粘附连接调节剂 P120-连环蛋白在视网膜图案形成中的作用。我们的结果表明，发育中的视网膜需要 P120-连环蛋白，这是该蛋白在下层后生动物上皮细胞中首次报道的发育功能。根据我们对 P120-Catenin 突变体的实时可视化以及遗传数据，我们得出结论，P120-Catenin 在眼睛发育过程中起到稳定 E-钙粘蛋白和粘附连接完整性的作用。由爱思唯尔爱尔兰有限公司出版
Correct patterning of cells within an epithelium is key to establishing their normal function. However, the precise mechanisms by which individual cells arrive at their final developmental niche remains poorly understood. We developed an optimized system for imaging the developing Drosophila retina, an ideal tissue for the study of cell positioning. Using this technique, we characterized the cellular dynamics of developing wild-type pupal retinas. We also analyzed two mutants affecting eye patterning and demonstrate that cells mutant for Notch or Roughest signaling were aberrantly dynamic in their cell movements. Finally, we establish a role for the adherens junction regulator P120-Catenin in retinal patterning through its regulation of normal adherens junction integrity. our results indicate a requirement for P120-Catenin in the developing retina, the first reported developmental function of this protein in the epithelia of lower metazoa. Based upon our live visualization of the P120-Catenin mutant as well as genetic data, we conclude that P120-Catenin is acting to stabilize E-cadherin and adherens junction integrity during eye development. Published by Elsevier Ireland Ltd.