Risungbinella pyongyangensis gen. nov., sp.. nov., a mesophilic member of the family Thermoactinomycetaceae isolated from an agricultural soil sample
Risungbinella pyongyangensis gen. nov., sp.. nov., a mesophilic member of the family Thermoactinomycetaceae isolated from an agricultural soil sample
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平壤 Risungbinella gen.
DOI：
10.1099/ijs.0.000328
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发表时间：
2015-08-01
影响因子：
2.8
通讯作者：
Peng, Fang
中科院分区：
文献类型：
--
作者：
Kim, MyongChol;Kim, TongRyul;Peng, Fang
从朝鲜民主主义人民共和国平壤的农业土壤样品中分离出一种被设计为 MC 210(T) 的嗜温菌株，并通过多相方法研究了其分类地位。该新菌株在PYI培养基上生长良好，且不产生扩散色素。最适生长温度为37℃。气生菌丝体发育良好，但不破碎。该菌株为革兰氏反应阳性且不活动，并在气生菌丝体上形成内生孢子。基于16S rRNA基因序列的系统发育分析表明菌株MC 210(T)属于Thermoactinomycetaceae家族。菌株MC 210(T)与地热微生物(Geothermomicrobium terrae)和Shimazuella kribbensis的典型菌株的16S rRNA基因序列相似性分别为92.90%和92.54%。菌株MC 210(T)的细胞壁含有内消旋二氨基庚二酸、谷氨酸和丙氨酸作为诊断氨基酸，全细胞水解产物含有葡萄糖、阿拉伯糖和半乳糖。菌株MC 210(T)含有反异-C-13:(0)、异-C-14:0 C-14:0、反异-C-15:0、C-16:0和异-C-13:0作为主要细胞脂肪酸。主要极性脂质为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂酰甘油、二磷脂酰甘油、两种未知氨基磷脂、一种未知氨基脂质、三种未知磷脂和五种未知极性脂质。主要的甲基萘醌是MK-7。基因组DNA的G+C含量为42.1mol%。根据我们的系统发育、生理和化学分类学数据，菌株 MC 210(T) 被认为代表了一个新的属和种，我们建议将其命名为 Risungbinella pyongyangensis gen。十一月，SP。 11 月，属于Thermoactinomycetaceae 科。 Risungbinella pyongyangensis 的模式菌株是 MC 210(T) (CCTCC AA 2013021(T)= NRRL B-591 18(T))
A mesophilic strain, designed MC 210(T), was isolated from an agricultural soil sample from Pyongyang, Democratic People's Republic of Korea, and its taxonomic position was investigated by using a polyphasic approach. The novel strain grew well on PYI medium, and no diffusible pigments were produced. The optimum temperature for growth was 37 degrees C. The aerial mycelium was well developed, but not fragmented. The strain was Gram-reaction-positive and non-motile and formed endospores on the aerial mycelium. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MC 210(T) belongs to the family Thermoactinomycetaceae. Strain MC 210(T) showed 16S rRNA gene sequence similarities of 92.90 and 92.54 % to the type strains of Geothermomicrobium terrae and Shimazuella kribbensis, respectively. The cell wall of strain MC 210(T) contained meso-diaminopimelic acid, glutamic acid and alanine as the diagnostic amino acids, and whole-cell hydrolysates contained glucose, arabinose and galactose. Strain MC 210(T) contained anteiso-C-13: (0), iSO-C-14:0 C-14:0, anteiso-C-15:0, C-16:0 and iso-C-13:0 as the major cellular fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown aminophospholipids, an unknown aminolipid, three unknown phospholipids and five unknown polar lipids. The predominant menaquinone was MK-7.The G+C content of the genomic DNA was 42.1 mol%. On the basis of our phylogenetic, physiological and chemotaxonomic data, strain MC 210(T) is considered to represent a novel genus and species, for which we propose the name Risungbinella pyongyangensis gen. nov., sp. nov., in the family Thermoactinomycetaceae. The type strain of Risungbinella pyongyangensis is MC 210(T) (CCTCC AA 2013021(T)= NRRL B-591 18(T))