Putative virulence-related genes in Vibrio anguillarum identified by random genome sequencing
Putative virulence-related genes in Vibrio anguillarum identified by random genome sequencing
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DOI：
10.1111/j.1365-2761.2006.00692.x
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发表时间：
2006-03-01
影响因子：
2.5
通讯作者：
Aoki, T
中科院分区：
文献类型：
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作者：
Rodkhum, C;Hirono, I;Aoki, T
鳗弧菌菌株H775-3的基因组部分通过随机测序程序确定。利用BLAST算法对2300个克隆（质粒文库2100个，cosmid文库200个）进行测序和同源性检索。测序克隆总长度为1.5 Mbp。核苷酸序列被划分为17大类功能。共鉴定出40个毒力相关基因，其中36个为新发现基因，包括毒素基因簇重复序列、溶血素基因、肠杆菌蛋白基因、蛋白酶基因、脂多糖生物合成基因、荚膜生物合成基因、鞭毛基因和菌毛基因。
The genome of Vibrio anguillarum strain H775-3 was partially determined by a random sequencing procedure. A total of 2300 clones, 2100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence-related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes.