Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle
Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle
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DOI：
10.1128/cdli.8.3.571-578.2001
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发表时间：
2001-05-01
期刊：
影响因子：
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通讯作者：
Hewinson, RG
中科院分区：
文献类型：
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作者：
Vordermeier, HM;Whelan, A;Hewinson, RG
在英国，政府的一项独立科学审查得出结论，开发牛分枝杆菌感染的牛疫苗对英国牛群的结核病控制具有最佳的长期前景。接种疫苗的先决条件是开发补充诊断试验，以区分接种疫苗的动物和感染分枝杆菌的动物，因此，检测和基于屠宰的控制策略可以与疫苗接种一起继续。卡介苗（BCG）是迄今为止唯一可用的预防结核病的疫苗。然而，基于结核菌素纯化蛋白衍生物的测试不能区分M。因此，牛支原体表达而BCG不表达的特异性抗原是鉴别诊断试剂的主要候选者。近年来，ESAT-6和CFP-10两种牛支原体抗原已被报道可作为牛支原体感染的诊断试剂。将这些肽中的五种与衍生自ESAT-6的五种肽一起配制成肽混合物。检测到牛感染的动物，而卡介苗接种或鸟分枝杆菌致敏的动物没有反应。使用自然感染的田间反应牛确定肽混合物作为全血γ干扰素测定中的抗原的灵敏度，并且使用来自BCG接种和未感染、未接种动物的血液确定特异性。在确诊结核病的牛中，发现该测定的灵敏度为77.9%，在BCG接种或未接种动物中的特异性为100%，这与在未感染或接种动物中检测结核菌素的特异性相比是有利的。总之，我们的结果表明，这种肽鸡尾酒可以区分M，牛感染和BCG疫苗接种具有高度的敏感性和特异性。
In Great Britain an independent scientific review for the government has concluded that the development of a cattle vaccine against Mycobacterium bovis infection holds the best long-term prospect for tuberculosis control in British herds, A precondition for vaccination is the development of a complementary diagnostic test to differentiate between vaccinated animals and those infected with M, bovis so that testing and slaughter-based control strategies can continue alongside vaccination. To date bacillus Calmette-Guerin (BCG), an attenuated strain of M, bovis, is the only available vaccine for the prevention of tuberculosis. However, tests based on tuberculin purified protein derivative cannot distinguish between M. bovis infection and BCC vaccination, Therefore, specific antigens expressed by M, bovis but absent from BCG constitute prime candidates for differential diagnostic reagents. Recently, two such antigens, ESAT-6 and CFP-10, have been reported to be promising candidates as diagnostic reagents for the detection of M, bovis infection in cattle, Here we report the identification of promiscuous peptides of CFP-10 that were recognized by M, bovis-infected cattle. Five of these peptides were formulated into a peptide cocktail together with five peptides derived from ESAT-6, Using this peptide cocktail in T-cell assays, M. bovis-infected animals were detected, while BCG-vaccinated or Mycobacterium avium-sensitized animals did not respond. The sensitivity of the peptide cocktail as an antigen in a whole-blood gamma interferon assay was determined using naturally infected field reactor cattle, and the specificity was determined using blood from BCG-vaccinated and noninfected, nonvaccinated animals. The sensitivity of the assay in cattle with confirmed tuberculosis was found to be 77.9%, with a specificity of 100% in BCG-vaccinated or nonvaccinated animals, This compares favorably with the specificity of tuberculin when tested in noninfected or vaccinated animals. In summary, our results demonstrate that this peptide cocktail can discriminate between M, bovis infection and BCG vaccination with a high degree of sensitivity and specificity.