SINGLE MICROTUBULES FROM SQUID AXOPLASM SUPPORT BIDIRECTIONAL MOVEMENT OF ORGANELLES
SINGLE MICROTUBULES FROM SQUID AXOPLASM SUPPORT BIDIRECTIONAL MOVEMENT OF ORGANELLES
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DOI：
10.1016/0092-8674(85)90160-6
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
REESE, TS
中科院分区：
文献类型：
--
作者：
SCHNAPP, BJ;VALE, RD;REESE, TS
单丝，从鱿鱼巨轴突的挤压轴质和可视化的视频增强微分干涉显微镜，运输细胞器双向。沿沿着同一条运输丝向相同或相反方向运动的细胞器可以相互通过而不发生碰撞，这表明每条运输丝都有几条用于细胞器运动的轨道。为了表征运输丝，首先通过视频显微镜检查细胞器的运动，然后将相同的丝在快速冷冻、冷冻干燥和旋转阴影后通过EM检查。支持细胞器双向运动的运输丝直径为22 nm-27 nm，具有指示单个微管的亚结构。免疫荧光显示，几乎所有的运输丝含有微管蛋白。单个微管可以作为细胞器运动的基质，并表明细胞器和微管之间的相互作用是快速轴突运输的基础。
Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by video-enhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were 1st examined by video microsocpy, and then the same filaments were examined by EM after rapid-freezing, freeze-drying and rotary-shadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm-27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. Single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.