SPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in biofluids
SPE-IMS-MS: An automated platform for sub-sixty second surveillance of endogenous metabolites and xenobiotics in biofluids
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DOI：
10.1016/j.clinms.2016.11.002
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发表时间：
2016-12-01
影响因子：
2.1
通讯作者：
Baker, Erin S.
中科院分区：
文献类型：
--
作者：
Zhang, Xing;Romm, Michelle;Baker, Erin S.
内源性代谢物和外源性物质的表征对于消除疾病的遗传和环境原因至关重要。然而，在大型队列中监测化学品暴露和疾病相关变化需要一个分析平台，该平台可提供快速测量、高灵敏度、有效分离、宽动态范围以及适用于广阔的化学空间。在这里，我们提出了一种新的小分子分析平台，通过将固相萃取与离子迁移谱和质谱（SPE-IMS-MS）相结合来满足这些要求。该平台能够以高重现性（CV）对人体生物流体中的内源性代谢物和外源性物质进行靶向和全局测量
Characterization of endogenous metabolites and xenobiotics is essential to deconvoluting the genetic and environmental causes of disease. However, surveillance of chemical exposure and disease-related changes in large cohorts requires an analytical platform that offers rapid measurement, high sensitivity, efficient separation, broad dynamic range, and application to an expansive chemical space. Here, we present a novel platform for small molecule analyses that addresses these requirements by combining solid-phase extraction with ion mobility spectrometry and mass spectrometry (SPE-IMS-MS). This platform is capable of performing both targeted and global measurements of endogenous metabolites and xenobiotics in human biofluids with high reproducibility (CV