Distribution of growth-associated class I alpha-tubulin and class II beta-tubulin mRNAs in adult rat brain.
Distribution of growth-associated class I alpha-tubulin and class II beta-tubulin mRNAs in adult rat brain.
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成年大鼠脑中生长相关的 I 类 α 微管蛋白和 II 类 β 微管蛋白 mRNA 的分布。
DOI：
10.1002/cne.903620306
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Oblinger,MM
中科院分区：
文献类型：
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作者：
Paden,CM;Zhou,X;Watt,JA;Burton,R;Pickett,J;Oblinger,MM
为了确定这些未成熟的微管蛋白同型mrna在成年大鼠脑中的持续表达程度，我们使用原位杂交技术对I类α‐微管蛋白（αl）和II类β‐微管蛋白（βII） mrna进行了全面的研究。观察到两种同型mrna的定性分布相似，两种探针的杂交强度在不同脑区明显不同。大脑中各种结构的神经元表现出强烈的杂交信号。虽然存在大量具有中等杂交强度的神经元可以解释小脑和齿状颗粒层等某些区域相对较高的总结合水平，但在大多数情况下，较高的区域mRNA水平反映了每个神经元更大的杂交强度。在整个大脑特定细胞核内的单个细胞之间杂交强度的差异很小。偶尔出现的分散在基底神经节上的高强度标记神经元为这种模式提供了最显著的例外。虽然α - 1‐微管蛋白和β - ii‐微管蛋白mRNA的分布没有质性差异，但α - 1‐微管蛋白和β - ii‐微管蛋白mRNA杂交的相对强度在一些脑区存在明显差异。胶质细胞的表达似乎对α - 1‐微管蛋白或β - ii‐微管蛋白mRNA的可检测水平没有显著贡献。这些发现表明，生长相关微管蛋白同型mrna的持续表达可能在成人大脑的特定神经元群中具有功能意义。本文讨论了α - 1‐和β - ii‐微管蛋白mRNA与GAP‐43 mRNA分布之间的部分重叠，以及生长相关微管蛋白基因表达在支持成人大脑细胞骨架周转、反应性轴突生长和树突重塑中的潜在作用。©1995 Wiley‐Liss Inc。
A comprehensive survey of class I α‐tubulin (αl) and class II β‐tubulin (βII) mRNAs was performed using in situ hybridization in order to determine the extent of continued expression of these immature tubulin isotype mRNAs in the adult rat brain. Qualitatively similar distributions of the two isotype mRNAs were observed, with marked variations in hybridization intensity of both probes apparent across different brain regions. Neurons in a wide variety of structures throughout the brain exhibited intense hybridization signals. While the presence of large numbers of neurons with a moderate hybridization intensity could account for the relatively high level of total binding in some regions such as the cerebellar and dentate granule layers, in most cases higher regional mRNA levels reflected greater hybridization intensity per neuron. Little variability in hybridization intensity was typically seen between individual cells within specific nuclei throughout the brain. The presence of occasional intensely labeled neurons scattered throughout the basal ganglia provided the most striking exception to this pattern. While no qualitative differences between the distributions of αl‐tubulin and βII‐tubulin mRNAs were observed, consistent differences in the relative intensity of hybridization for α1‐tubulin versus βII‐tubulin mRNA were apparent in a few brain regions. Expression by glia did not appear to contribute significantly to detectable levels of either α1‐tubulin or βII‐tubulin mRNA. These findings suggest that continued expression of growth‐associated tubulin isotype mRNAs may have functional significance in specific neuronal populations of the adult brain. Partial overlap between the distributions of α1‐ and βII‐tubulin mRNAs and that of GAP‐43 mRNA is discussed, as are potential roles for growth‐associated tubulin gene expression in supporting cytoskeletal turnover, reactive axonal growth, and dendritic remodeling in the adult brain. © 1995 Wiley‐Liss Inc.