Nicotine treatment regulates neuropeptide S system expression in the rat brain
Nicotine treatment regulates neuropeptide S system expression in the rat brain
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DOI：
10.1016/j.neuro.2007.06.003
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发表时间：
2007-11-01
期刊：
影响因子：
3.4
通讯作者：
Lopez, Miguel
中科院分区：
文献类型：
--
作者：
Lage, Ricardo;Gonzalez, C. Ruth;Lopez, Miguel
尼古丁对睡眠、觉醒和体重有显著影响。然而，这些行动背后的神经机制尚未完全理解。神经肽S（Neuropeptide S，NPS）是近年来发现的一种调节睡眠、焦虑和摄食的神经肽。在这里，我们研究了急性和慢性尼古丁治疗对大鼠下丘脑和脑干中NPS-R及其受体（NPS-R）表达的影响，通过使用实时PCR。我们的研究结果表明，慢性尼古丁治疗引起的显着变化，而急性治疗专门诱导脑干中的NPS-R的mRNA水平显着增加的NPS-R的表达。有趣的是，我们没有发现下丘脑和脑干中其他基因的表达水平发生变化。总的来说，这些数据表明，尼古丁在大鼠下丘脑和脑干中特异性地调节神经递质系统。(c)2007爱思唯尔公司All rights reserved.
Nicotine has marked effects on sleep, arousal and body weight. However, the neuronal mechanisms underlying these actions are not fully understood. Neuropeptide S (NPS) is a recently discovered neuropeptide regulating sleep, anxiety and feeding. Here, we examined the effect of acute and chronic nicotine treatment on the expression of NPS and its receptor (NPS-R) in the hypothalamus and brainstem of rats by using real-time PCR. Our results showed that chronic nicotine treatment induced significant changes in NPS and NPS-R expression whereas acute treatment exclusively induces a marked increase in the mRNA levels of NPS-R in the brainstem. Interestingly, we detected no changes in the expression levels of other set of genes present both in hypothalamus and brainstem. Overall, these data suggest that NPS system is specifically regulated by nicotine in the rat hypothalamus and brainstem. (c) 2007 Elsevier Inc. All rights reserved.