Structural Proximity of Mitochondria to Calcium Release Units in Rat Ventricular Myocardium May Suggest a Role in Ca2+ Sequestration
Structural Proximity of Mitochondria to Calcium Release Units in Rat Ventricular Myocardium May Suggest a Role in Ca2+ Sequestration
复制标题
DOI：
10.1111/j.1749-6632.1998.tb08295.x
复制
发表时间：
1998-09
影响因子：
5.2
通讯作者：
V. Ramesh;V. Sharma;Shey-Shing Sheu;Clara Franzini-Armstrong
中科院分区：
文献类型：
--
作者：
V. Ramesh;V. Sharma;Shey-Shing Sheu;Clara Franzini-Armstrong
方法取4-6周龄成年SD大鼠左、右心室，戊二醛和锇酸固定，醋酸双氧铀整体染色。测量以下距离：近（NFD）和远（FFD）脚距离是线粒体膜与相邻CRU内最近和最远脚（RyR）之间的最小距离。中心距（CD）是线粒体到T小管中心的最小距离。（N= 100个测量值，来自3只大鼠）。线粒体在肌原纤维之间密集地堆积成连续的行，并且CRU主要位于两个伸长的纵向取向的线粒体的末端之间（图1）。大多数线粒体图谱（122/194或63%）与CRU非常接近，超过90%的CRU与线粒体非常接近。两个细胞器之间的实际接触可能比这个数字更频繁，因为许多细胞器不在截面上（图1，C）。近和远脚距离的平均值为33和188 nm（表1）。T小管的中心与CRU中的所有通道大致等距，也紧邻线粒体（138-160 nm）。
METHODSLeft and right ventricles of adult (4-6 wk old) Sprague-Dawley rats were fixed in glutaraldehyde and osmium and stained en bloc in uranyl acetate. The following distances were measured: Near (NFD) and far (FFD) feet distances, are the smallest distances between the mitochondrial membrane and the nearest and farthest feet (RyRs) within an adjacent CRU. Center distance (CD) is the smallest mitochondria to center of T tubule distance.(N= 100 measurements from 3 rats).RESULTSMitochondria are densely packed in continuous rows between the myofibrils and CRUs are mostly nestled between the ends of two elongated, longitudinally oriented mitochondria (Fig. 1). Most of the mitochondrial profiles (122 over 194 or 63%) are in close proximity of a CRU and over 90% CRUs are in close proximity to a mitochondrion. The actual contacts between the two organelles are probably more frequent than this number, since many are not in the plane of section (Fig 1, C). Near and far feet distances are an average of 33 and 188 nm (Table 1). The center of the T tubule, approximately equidistant from all the channels in the CRU, is also in close proximity to the mitochondria (138-160 nm).