Isolation, structural characterization and antioxidant activity of a neutral polysaccharide from Sisal waste

Isolation, structural characterization and antioxidant activity of a neutral polysaccharide from Sisal waste
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DOI:
10.1016/j.foodhyd.2013.12.012
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发表时间:
2014-08
期刊:
影响因子:
10.7
通讯作者:
Xuehong Zhang;Lina Liu;Cuiwu Lin
Xuehong Zhang;Lina Liu;Cuiwu Lin
中科院分区:
农林科学1区
文献类型:
--
作者:
Xuehong Zhang;Lina Liu;Cuiwu Lin

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从剑麻(Agave sisalana)植物纤维提取后的废料中分离得到一种新的水溶性多糖(PSP)。单糖组成分析表明,PSP由半乳糖、葡萄糖、甘露糖、鼠李糖、阿拉伯糖和半乳糖醛酸组成,摩尔比为3.0:2.4:1.4:1.0:0.2:0.2。结构分析表明,PSP主链由1,4-连接的β-D-Galp、1,3-连接的β-D-Glcp、1,3-连接的α-D-Manp和1,2-连接的α-L-Rhapin组成,比例为3:1:1:1。主链中的一些Glcp、Manp和Rhap残基是分支的。体外抗氧化活性测定表明,PSP以剂量依赖性方式适度清除羟基和DPPH自由基。
A new water-soluble polysaccharide (PSP) was isolated from the waste of a Sisal (Agave sisalana) plant after the plant's fibers were extracted. The monosaccharide composition analysis showed that PSP consists of galactose, glucose, mannose, rhamnose, arabinose, and galacturonic acid in a molar ratio of 3.0:2.4:1.4:1.0:0.2:0.2. The structure analysis indicated that the PSP backbone chain comprises 1,4-linked β-D-Galp; 1,3-linked β-D-Glcp; 1,3-linked α-D-Manp; and 1,2-linked α-L-Rhapin a ratio of 3:1:1:1. Some of the Glcp, Manp, and Rhapresidues in the backbone chain were branched. The in vitro antioxidant activity assay showed that PSP moderately scavenges hydroxyl and DPPH radicals in a dose-dependent manner.