NMR Metabolic Fingerprinting Based Identification of Grapevine Metabolites Associated with Downy Mildew Resistance
NMR Metabolic Fingerprinting Based Identification of Grapevine Metabolites Associated with Downy Mildew Resistance
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DOI：
10.1021/jf902069f
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发表时间：
2009-10-28
影响因子：
6.1
通讯作者：
Verpoorte, Robert
中科院分区：
文献类型：
--
作者：
Ali, Kashif;Maltese, Federica;Verpoorte, Robert
葡萄(Vitis vinifera SSP.)(Vinifera L.)葡萄因其众多的营养价值和影响健康的活动而被广泛研究。在本研究中，基于核磁共振H-1波谱和多元数据分析的粗叶提取物代谢产物指纹图谱，对6个不同葡萄品种的代谢特性进行了研究，其中包括抗霜霉病和白粉病的‘Regent’和感病的‘Lemberger’等。还使用了几种二维(2D)-核磁共振技术，从而鉴定了一些不同类型的化合物。对处理后的核磁共振数据进行主成分分析(PCA)、系统聚类分析(HCA)和偏最小二乘判别分析(PLS-DA)，结果表明品种间存在明显差异。负责葡萄品种鉴别的代谢产物主要有有机酸、氨基酸、碳水化合物、苯丙烷类和类黄酮类。还根据品种对霜霉病的抗性进行了区分，并鉴定了与这一特性相关的代谢产物，即槲皮素-3-O-葡萄糖苷和反式阿魏酰衍生物。在此基础上，给出了不同植物代谢产物在不同葡萄品种间的分布。
Grapevine (Vitis vinifera ssp. vinifera L.) and grapes have been extensively studied due to their numerous nutritional benefits and health affecting activities. In this study, metabolite fingerprinting of crude leaf extracts, based on H-1 nuclear magnetic resonance (NMR) spectroscopy and multivariate data analyses, has been used for the metabolic characterization of six different grapevine cultivars including downy and powdery mildew resistant 'Regent' and susceptible 'Lemberger' among others. Several two-dimensional (2D)-NMR techniques were also employed leading to the identification of a number of different types of compounds. Principal component analysis (PCA), hierarchical cluster analysis (HCA), and partial least-squares-discriminant analysis (PLS-DA) of the processed 1H NMR data revealed clear differences among the cultivars. Metabolites responsible for the discrimination in different grapevine cultivars belong to major classes, that is, organic acids, amino acids, carbohydrates, phenylpropanoids and flavonoids. A differentiation of the cultivars based on their resistance to downy mildew infection was also achieved, and metabolites associated with this trait, namely, quercetin-3-O-glucoside and a trans-feruloyl derivative, were identified. On the basis of these results, the distribution of different plant metabolites among the different grapevine cultivars is presented.