The first genetic linkage map of Luohanguo (Siraitia grosvenorii ) based on ISSR and SRAP markers.
The first genetic linkage map of Luohanguo (Siraitia grosvenorii ) based on ISSR and SRAP markers.
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DOI：
10.1139/g10-084
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发表时间：
2011
期刊：
影响因子：
3.1
通讯作者：
Lihua Liu;Xiaojun Ma;Jianhe Wei;Jiaming Qin;C. Mo
中科院分区：
文献类型：
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作者：
Lihua Liu;Xiaojun Ma;Jianhe Wei;Jiaming Qin;C. Mo
本研究首次构建了罗汉果（Siraitia grosvenorii（Swingle）C.利用简单重复序列（intersimple sequence repeat，ISSR）和序列相关扩增多态性（sequence related amplified polymorphism，SRAP）标记，对150个F_1群体个体构建了F_1群体的SSR标记。100个ISSRs和196个SRAP引物组合分别扩增出51个和222个多态性标记。在获得的273个标记中，199个标记（29个ISSR和170个SRAP）被定位到25个连锁群。该图谱覆盖1463.3cM，相邻标记之间的平均图距为7.35cM，两个标记之间的最大图距为52.6cM。标记随机分布在25个连锁群中，仅在连锁群的远端区域有少量聚集。25个连锁群由2-36个位点组成，连锁群长度为19.5 ~ 152.6cM，占总图距的59.8%。该图谱为罗汉果的分子育种工作提供了参考。
In this study, the first genetic map of Luohanguo (Siraitia grosvenorii (Swingle) C. Jeffrey) was constructed with 150 F₂ population individuals using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. A total of 100 ISSRs and 196 SRAP primer combinations generated 51 and 222 polymorphic markers, respectively. Among the 273 markers obtained, 199 markers (29 ISSRs and 170 SRAPs) were mapped to 25 linkage groups. The map covered 1463.3 cM with a mean map distance of 7.35 cM between adjacent markers and a maximum map distance of 52.6 cM between two markers. The markers were distributed randomly in 25 groups except for minor clusters in the distal region of linkage groups. All 25 linkage groups consisted of 2-36 loci ranging in length from 19.5 to 152.6 cM and accounted for 59.8% of the total map distance. This map provides reference information for future molecular breeding work on Luohanguo.