Synthesis of nucleobase-caged peptide nucleic acids having improved photochemical properties
Synthesis of nucleobase-caged peptide nucleic acids having improved photochemical properties
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具有改善的光化学性质的核碱基笼肽核酸的合成
DOI：
10.1039/c4ob00418c
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
T. Furuta
中科院分区：
文献类型：
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作者：
T. Watanabe;T. Hoshida;J. Sakyo;M. Kishi;S. Tanabe;J. Matsuura;S. Akiyama;M. Nakata;Y. Tanabe;A. Suzuki;S. Watanabe;T. Furuta
新合成了一种含(6-bromo-7-methoxycoumarin)-4-ylmethoxycarbonyl(Bmcmoc)环化基团的碱基笼状多肽核酸。将Bmcmoc包埋的PNA进行光解，制得具有较高光化学效率的母体PNA。单个Bmcmoc基团的引入足以抑制聚合酶链式反应(PCR)的钳制活性和三链侵袭复合体的形成。光介导法还恢复了PCR的钳制活性。
A nucleobase-caged peptide nucleic acid (PNA) having a (6-bromo-7-methoxycoumarin)-4-ylmethoxycarbonyl (Bmcmoc) caging group was newly synthesized. The Bmcmoc-caged PNAs were photolyzed to produce parent PNAs with a high photochemical efficiency. Introduction of a single Bmcmoc group was sufficient to suppress polymerase chain reaction (PCR) clamping activity and triplex invasion complex formation. Photo-mediated restoration of the PCR clamping activity was also demonstrated.