A one-step method for protein estimation in biological samples: Nitration of tyrosine in nitric acid
A one-step method for protein estimation in biological samples: Nitration of tyrosine in nitric acid
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DOI：
10.1006/abio.1998.2980
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发表时间：
1999-02-01
影响因子：
2.9
通讯作者：
Kaufmann, SH
中科院分区：
文献类型：
--
作者：
Bible, KC;Boerner, SA;Kaufmann, SH
通常采用多种方法来测定生物样品中的蛋白质。不幸的是，作为生物缓冲液成分的几种化合物会干扰这些方法，限制了它们的应用。先前的研究表明，酪氨酸在硝酸中快速硝化，生成 3-硝基酪氨酸，其 lambda(max) 为 358 nm。利用这种反应，我们开发了一种用于评估生物样品中蛋白质含量的一步法。常见的干扰物质，包括 SDS、尿素、甘油、硫酸铵和 β-巯基乙醇，不会干扰该方法。由于其简单性，该反应可能有助于估计各种生物样品中的蛋白质含量，(C) 1999 年学术出版社。
A number of methods are commonly employed for the determination of protein in biological samples. Unfortunately, several compounds that are constituents of biological buffers interfere with these methods, limiting their application. Previous studies have demonstrated that tyrosine rapidly undergoes nitration in nitric acid to yield 3-nitrotyrosine, which has a lambda(max) of 358 nm. Utilizing this reaction, we have developed a one-step method for the assessment of protein content in biological samples. Common interfering substances, including SDS, urea, glycerol, ammonium sulfate, and beta-mercaptoethanol, do not interfere with this method. Because of its simplicity, this reaction might be useful for estimating protein content in a variety of biological samples, (C) 1999 Academic Press.