On the monophyly of chromalveolates using a six-protein phylogeny of eukaryotes
On the monophyly of chromalveolates using a six-protein phylogeny of eukaryotes
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DOI：
10.1099/ijs.0.63216-0
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发表时间：
2005-01-01
影响因子：
2.8
通讯作者：
Keeling, PJ
中科院分区：
文献类型：
--
作者：
Harper, JT;Waanders, E;Keeling, PJ
主要真核生物谱系的全球同源性是对分子生物遗传学的一个重大和持续的挑战。目前，有五个假设的主要谱系或真核生物的“超群”。其中之一，chromalveolates，代表了原生生物和藻类多样性的很大一部分。chromalveolate假说最初是基于在其许多成员中发现的光合细胞器（质体）之间的相似性，并得到了质体相关基因分析的支持。然而，由于质体可以在真核细胞谱系之间移动，因此重要的是从核-胞质宿主谱系产生的数据提供额外的支持。基因编码六种不同的胞质蛋白质从各种chromalveolates（产生68个新的基因序列）已被表征，使多个基因分析，包括所有六个主要谱系的chromalveolates，可以进行比较，并连接与数据代表所有五个假设的超组。与先前的分析相比，对大部分基因突变的总体支持率下降了，这些分析将较少的基因连接起来，以减少taya。然而，四个chromalveolate谱系（apicomplexans，纤毛虫，腰鞭毛虫和heterokonts）始终形成一个单系组合，而剩下的两个（隐藻和haptophytes）形成一个弱支持组。而这些结果是一致的单系性的chromalveolates从质体数据推断，测试这一假设将需要大量增加的数据，从各种各样的生物。
A global phylogeny of major eukaryotic lineages is a significant and ongoing challenge to molecular phylogenetics. Currently, there are five hypothesized major lineages or 'supergroups' of eukaryotes. One of these, the chromalveolates, represents a large fraction of protist and algal diversity. The chromalveolate hypothesis was originally based on similarities between the photosynthetic organelles (plastids) found in many of its members and has been supported by analyses of plastid-related genes. However, since plastids can move between eukaryotic lineages, it is important to provide additional support from data generated from the nuclear-cytosolic host lineage. Genes coding for six different cytosolic proteins from a variety of chromalveolates (yielding 68 new gene sequences) have been characterized so that multiple gene analyses, including all six major lineages of chromalveolates, could be compared and concatenated with data representing all five hypothesized supergroups. Overall support for much of the phylogenies is decreased over previous analyses that concatenated fewer genes for fewer taya. Nevertheless, four of the six chromalveolate lineages (apicomplexans, ciliates, dinoflagellates and heterokonts) consistently form a monophyletic assemblage, whereas the remaining two (cryptomonads and haptophytes) form a weakly supported group. Whereas these results are consistent with the monophyly of chromalveolates inferred from plastid data, testing this hypothesis is going to require a substantial increase in data from a wide variety of organisms.