Employing mated females as recipients for transfer of cloned dog embryos
Employing mated females as recipients for transfer of cloned dog embryos
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DOI：
10.1071/rd11221
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发表时间：
2013-01-01
影响因子：
1.9
通讯作者：
Lee, Byeong Chun
中科院分区：
文献类型：
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作者：
Kim, Geon A.;Oh, Hyun Ju;Lee, Byeong Chun
有研究表明，共移植孤雌生殖胚胎可以提高哺乳动物与克隆胚胎的妊娠成功率。作为共同移植单性生殖的替代方案，我们在狗身上使用了作为交配结果拥有体内受精胚胎的受体雌性，以确定交配的雌性是否适合克隆胚胎的受体。同时测定了交配受体对犬体细胞核移植胚胎着床率和妊娠率的影响。克隆的胚胎被移植到自然同步的雌性狗的输卵管中，这些雌性狗在排卵前与公狗交配。配对受者的妊娠率(50%)与非配对受者的妊娠率(28.57%；P>；0.05)相似。然而，交配受体的克隆幼崽接生率显著高于非交配受体(10.53比2.38%；P>；0.05)。交配受者在预产期前孕酮水平下降会导致自然分娩。然而，非交配受者的克隆幼崽是通过剖腹产出生的，因为这些雌性幼崽的孕酮浓度直到预产期才出现下降。本研究首次证明交配后的母犬可以作为克隆胚胎的受体。
It has been suggested that co-transferring parthenogenetic embryos could improve the pregnancy success rate with cloned embryos in mammals. As an alternative to co-transferring parthenotes, in dogs we employed recipient females that possessed in vivo-fertilised embryos as a result of mating to determine whether mated bitches could be suitable recipients for cloned embryos. The effect of using mated recipients on implantation and pregnancy rates of canine somatic cell nuclear transfer embryos was also determined. Cloned embryos were transferred into the oviducts of naturally synchronous females that had mated with male dogs before ovulation. The pregnancy rate appeared to be similar between mated recipients (50%) and non-mated recipients (28.57%; P > 0.05). However, the delivery rate of cloned pups was significantly higher in mated recipients than non-mated recipients (10.53 vs 2.38%; P > 0.05). A decrease in progesterone levels in the mated recipients before the due date induced natural delivery. However, cloned pups in non-mated recipients were delivered by Caesarean section because the fall in progesterone concentration in these females did not occur until the due date. The present study demonstrated for the first time that mated female dogs can be used as recipients for cloned embryos.