SARS Accessory Proteins ORF3a and 9b and Their Functional Analysis
SARS Accessory Proteins ORF3a and 9b and Their Functional Analysis
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DOI：
10.1007/978-3-642-03683-5_11
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发表时间：
2009-07-22
期刊：
影响因子：
--
通讯作者：
Sun B
中科院分区：
文献类型：
--
作者：
Lu W;Xu K;Sun B
SARS冠状病毒（CoV）正链RNA病毒基因组编码14个开放阅读框（ORF），其中8个编码被称为“辅助蛋白”的蛋白质。这些蛋白质帮助病毒感染宿主并提高毒力。在这一章中，我们描述了一些我们的最新研究的结构和功能的两个这样的辅助蛋白：ORF 3a和9 b。ORF 3a辅助蛋白是SARS-CoV中最大的辅助蛋白，是由三个跨膜结构域组成的独特的膜蛋白。它共定位于细胞膜和宿主高尔基体网络上，并可能参与感染过程中离子通道的形成。类似地，ORF 9 b辅助蛋白是98个氨基酸，与刺突蛋白和核衣壳蛋白缔合，并且具有不寻常的膜结合性质。在本章中，我们提出了这两种辅助蛋白可能的新作用，从长远来看，它们可能包含许多未解问题的答案，也为我们的药物和疫苗设计提供了新的思路。
The SARS coronavirus (CoV) positive-stranded RNA viral genome encodes 14 open reading frames (ORFs), eight of which encode proteins termed as “accessory proteins.” These proteins help the virus infect the host and promote virulence. In this chapter we describe some of our latest investigations into the structure and function of two such accessory proteins: ORF3a and 9b. The ORF3a accessory protein is the largest accessory protein in SARS-CoV and is a unique membrane protein consisting of three transmembrane domains. It colocalizes on the cell membrane and host Golgi networks and may be involved in ion channel formation during infection. Similarly the ORF9b accessory protein is 98 amino acids, associates with the spike and nucleocapsid proteins and has unusual membrane binding properties. In this chapter we have suggested possible new roles for these two accessory proteins which may in the long run contain answers to many unanswered questions and also give us new ideas for drugs and vaccine design.