Factors affecting freezing tolerance: a comparative transcriptomics study between field and artificial cold acclimations in overwintering evergreens
Factors affecting freezing tolerance: a comparative transcriptomics study between field and artificial cold acclimations in overwintering evergreens
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影响耐冻性的因素：越冬常绿植物田间和人工冷驯化的比较转录组学研究
DOI：
10.1111/tpj.14899
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发表时间：
2020-08-31
期刊：
影响因子：
7.2
通讯作者：
Xia, Yi-Ping
中科院分区：
文献类型：
--
作者：
Liu, Bing;Wang, Xiu-Yun;Xia, Yi-Ping
冷驯化是植物在冬季提高抗冻性的重要途径。全球变暖可能干扰CA，增加冬季冻害的可能性。因此，通过完整的CA开发强大的FT是必不可少的。为了探讨木本多年生植物CA的分子机制，我们比较了现场和人工CA。转录组学数据表明，光合/光保护和脂肪酸代谢途径在田间CA中特异性富集;碳水化合物代谢、次生代谢和昼夜节律途径在田间和人工CA中普遍富集。与室内营养生长的植物相比，秋季暖空气温度下的光信号可诱导植物叶片脱落酸（阿坝）和茉莉酸（JA）浓度的积累，激活植物体内Ca ~（2+）、阿坝和JA信号转导。随着冬季逐渐变冷，花色素苷的积累、叶绿素的降解、光系统II反应中心的关闭/降解以及葡萄糖和果糖的大量积累有助于在田间CA期间获得稳定的FT。此外，我们观察到杜鹃花‘Elsie Lee’，阿坝和JA在冬季下降，这可能是由于玉米黄质的快速散热和膜流动性的不饱和脂肪酸的强烈需求。总之，我们的研究结果表明，人工CA有局限性，以了解现场CA和现场光信号（如短光周期，光强度和/或光质量）之前，在秋季低温可能是必不可少的完整CA。
Cold acclimation (CA) is a well-known strategy employed by plants to enhance freezing tolerance (FT) in winter. Global warming could disturb CA and increase the potential for winter freeze-injury. Thus, developing robust FT through complete CA is essential. To explore the molecular mechanisms of CA in woody perennials, we compared field and artificial CAs. Transcriptomic data showed that photosynthesis/photoprotection and fatty acid metabolism pathways were specifically enriched in field CA; carbohydrate metabolism, secondary metabolism and circadian rhythm pathways were commonly enriched in both field and artificial CAs. When compared with plants in vegetative growth in the chamber, we found that the light signals with warm air temperatures in the fall might induce the accumulation of leaf abscisic acid (ABA) and jasmonic acid (JA) concentrations, and activate Ca2+, ABA and JA signaling transductions in plants. With the gradual cooling occurrence in winter, more accumulation of anthocyanin, chlorophyll degradation, closure/degradation of photosystem II reaction centers, and substantial accumulation of glucose and fructose contributed to obtaining robust FT during field CA. Moreover, we observed that inRhododendron'Elsie Lee', ABA and JA decreased in winter, which may be due to the strong requirement of zeaxanthin for rapid thermal dissipation and unsaturated fatty acids for membrane fluidity. Taken together, our results indicate that artificial CA has limitations to understand the field CA and field light signals (like short photoperiod, light intensity and/or light quality) before the low temperature in fall might be essential for complete CA.