Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue.
Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue.
复制标题
人ipsc来源的神经元和组织中病理性α-突触核蛋白聚集体的检测。
DOI：
10.1016/j.xpro.2021.100372
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Mazzulli JR
中科院分区：
文献类型：
--
作者：
Stojkovska I;Mazzulli JR
The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein. For complete details on the use and execution of this protocol, please refer to and. α-Synuclein aggregates can be characterized by sequential protein extraction Protocol is optimized for detecting α-synuclein in iPSC-derived neurons or brain tissue Gel filtration is a useful method for the detection of oligomeric intermediates Inclusions can be further analyzed by immunofluorescence and Thioflavin S staining The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein.
