Global Transcriptomic Analysis Reveals the Mechanism of Phelipanche aegyptiaca Seed Germination.
Global Transcriptomic Analysis Reveals the Mechanism of Phelipanche aegyptiaca Seed Germination.
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全球转录组分析揭示了埃及飞利浦种子萌发的机制
DOI：
10.3390/ijms17071139
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发表时间：
2016-07-15
影响因子：
5.6
通讯作者：
Zhao S
中科院分区：
文献类型：
--
作者：
Yao Z;Tian F;Cao X;Xu Y;Chen M;Xiang B;Zhao S
埃及艳苔是列当科最具破坏性的根寄生植物之一。这种植物对世界各地的作物产量有重大影响。条件和宿主根刺激剂，特别是独脚金内酯，需要独特的种子萌发。然而，由于基因组信息不足，尚未对这一现象进行广泛的研究。对埃及地锦萌发种子进行深度RNA测序，包括从头组装和功能注释。组装的转录组用于分析种子萌发过程中的转录动态。确定了涉及的关键基因类别。根据NR、GO、COG、KOG和KEGG数据库，共确定了274，964个转录本，并注释了53，921个单基因。总的来说，5324差异表达的基因休眠，条件，和GR24处理的种子进行了鉴定。GO和KEGG富集分析证明了与DNA、RNA和蛋白质修复和生物合成以及碳水化合物和能量代谢相关的许多DEG。阿坝和乙烯在此过程中起重要作用。GR24的应用导致阿坝和乙烯相关基因的显着变化。类胡萝卜素生物合成抑制剂氟立酮单独处理能诱导埃及地锦种子萌发。此外，埃及地锦的预处理可能不是其生长的必经阶段，因为预处理并没有上调与独脚金内酯信号转导相关的MAX2和KAI2基因的转录水平变化。
Phelipanche aegyptiaca is one of the most destructive root parasitic plants of Orobanchaceae. This plant has significant impacts on crop yields worldwide. Conditioned and host root stimulants, in particular, strigolactones, are needed for unique seed germination. However, no extensive study on this phenomenon has been conducted because of insufficient genomic information. Deep RNA sequencing, including de novo assembly and functional annotation was performed on P. aegyptiaca germinating seeds. The assembled transcriptome was used to analyze transcriptional dynamics during seed germination. Key gene categories involved were identified. A total of 274,964 transcripts were determined, and 53,921 unigenes were annotated according to the NR, GO, COG, KOG, and KEGG databases. Overall, 5324 differentially expressed genes among dormant, conditioned, and GR24-treated seeds were identified. GO and KEGG enrichment analyses demonstrated numerous DEGs related to DNA, RNA, and protein repair and biosynthesis, as well as carbohydrate and energy metabolism. Moreover, ABA and ethylene were found to play important roles in this process. GR24 application resulted in dramatic changes in ABA and ethylene-associated genes. Fluridone, a carotenoid biosynthesis inhibitor, alone could induce P. aegyptiaca seed germination. In addition, conditioning was probably not the indispensable stage for P. aegyptiaca, because the transcript level variation of MAX2 and KAI2 genes (relate to strigolactone signaling) was not up-regulated by conditioning treatment.