Extracellular cysteine proteinase and collagenase activities as a consequence of tumor-host interaction in the rabbit V2 carcinoma.
Extracellular cysteine proteinase and collagenase activities as a consequence of tumor-host interaction in the rabbit V2 carcinoma.
复制标题
兔 V2 癌中肿瘤与宿主相互作用导致的细胞外半胱氨酸蛋白酶和胶原酶活性。
DOI：
--
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
P. Sträuli
中科院分区：
文献类型：
--
作者：
A. Baici;M. Gyger;P. Sträuli
将兔V2癌标本保持在器官培养中以研究蛋白酶的分泌。弹性蛋白酶样、糜蛋白酶样、纤溶酶原激活物样、组织蛋白酶B样和胶原酶活性用灵敏的荧光技术测定。在这些酶中，在肿瘤组织的原代培养物中大量分泌的唯一活性是胶原酶和类似于组织蛋白酶B的半胱氨酸蛋白酶。腹腔内生长的肿瘤和正常的皮下组织的兔子的共培养物导致显着更高的生产的半胱氨酸蛋白酶和胶原酶相比，单独的组织的活动的总和。荷瘤兔皮下组织块分泌的半胱氨酸蛋白酶和胶原酶明显高于正常动物。与对照组相比，用肿瘤条件培养基刺激的正常皮下组织的外植体分泌更高量的两种酶。半胱氨酸蛋白酶在某些性质上与兔肝组织蛋白酶B相似，但来自肿瘤-宿主系统的酶对中等碱性pH显示出显著的稳定性。我们认为，来自肿瘤或迁移细胞的扩散因子促进宿主中胶原酶和半胱氨酸蛋白酶的合成和分泌增加，并且这两种酶在V2癌侵袭周围组织的过程中可能发挥协同作用。
Specimens of the rabbit V2 carcinoma were maintained in organ culture to study the secretion of proteinases. Elastase-like, chymotrypsin-like, plasminogen activator-like, cathepsin B-like and collagenase activities were assayed with sensitive fluorimetric techniques. Of these enzymes, the only activities that were secreted in considerable amounts in primary cultures of tumor tissue were collagenase and a cysteine proteinase resembling cathepsin B. Co-cultures of intraperitoneally grown tumor and normal subcutaneous tissue of the rabbit resulted in significantly higher production of the cysteine proteinase and collagenase compared to the sum of the activities of the separate tissues. Explants of subcutaneous tissue of tumor-bearing rabbits secreted significantly more cysteine proteinase and collagenase than explants from normal animals. Explants from normal subcutaneous tissue stimulated with tumor-conditioned culture medium secreted both enzymes in higher amounts compared to the controls. The cysteine proteinase was similar in some properties to rabbit liver cathepsin B, but the enzyme from the tumor-host system showed a remarkable stability to a moderately alkaline pH. We suggest that a diffusible factor, derived from the tumor or immigrated cells, promotes an increased synthesis and secretion of collagenase and cysteine proteinase in the host, and that both enzymes may play cooperative roles during invasion of the surrounding tissues by the V2 carcinoma.