Distribution of Amelotin in Mouse Tooth Development
Distribution of Amelotin in Mouse Tooth Development
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釉质在小鼠牙齿发育中的分布
DOI：
10.1002/ar.21022
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Han, Tingting
中科院分区：
文献类型：
--
作者：
Gao, Yuguang;Wang, Wanchun;Han, Tingting
釉质由成釉细胞在牙齿发育过程中表达和分泌，但釉蛋白在釉质发育过程中的分布尚不清楚。在这篇报道中，我们首先用免疫组织化学的方法研究了釉质蛋白在发育中牙齿中的表达。在釉质发育的分泌期和成熟期，釉质基质中可检测到Amelotin。在分泌性成釉细胞顶端的Tomes‘s突起也可观察到Amelotin。然后，我们通过RT-PCR将成釉蛋白基因与成釉蛋白、成釉蛋白和成釉蛋白在出生后小鼠下颌骨中的表达进行了比较。釉质蛋白的表达早在出生后第0天的下颌骨就已检测到，并在牙齿发育过程中与釉原蛋白、成釉蛋白和釉蛋白共表达。这些数据有力地表明，釉质是一种釉质基质蛋白，在釉质发育的分泌和成熟阶段表达。Anat Rec，2010年。©2009威利-利斯，Inc.
Amelotin is expressed and secreted by ameloblasts in tooth development, but amelotin distribution during enamel development is not clear. In this report, we first investigated amelotin expression in developing teeth by immunohistochemistry. Amelotin was detected in the enamel matrix at the secretion and maturation stages of enamel development. Amelotin was also observed at Tomes' processes on the apical ends of secretory ameloblasts. We then compared amelotin gene expression with those of amelogenin, enamelin, and ameloblastin in the mandibles of postnatal mice by RT‐PCR. The expression of amelotin was detected as early as in postnatal day 0 mandibles and amelotin was coexpressed with amelogenin, ameloblastin, and enamelin during tooth development. These data strongly suggest that amelotin is an enamel matrix protein expressed at the secretion and maturation stages of enamel development. Anat Rec, 2010. © 2009 Wiley‐Liss, Inc.