Detection of Thy‐1, T‐200, Lyt‐1 and Lyt‐2‐bearing cells in the developing lymphoid organs of the mouse embryo in vivo and in vitro
Detection of Thy‐1, T‐200, Lyt‐1 and Lyt‐2‐bearing cells in the developing lymphoid organs of the mouse embryo in vivo and in vitro
复制标题
体内和体外小鼠胚胎发育中淋巴器官中 Thy-1、T-200、Lyt-1 和 Lyt-2 携带细胞的检测
DOI：
10.1002/eji.1830120403
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发表时间：
1982
影响因子：
5.4
通讯作者：
J. Owen
中科院分区：
文献类型：
--
作者：
W. van Ewijk;E. Jenkinson;J. Owen
本文用冰冻切片免疫过氧化物酶试验和细胞悬液双色免疫荧光试验中的单抗，研究了体内外胚胎胸腺淋巴样细胞上细胞膜抗原的出现。研究表明，在个体发育的最早阶段，细胞对Lyt抗原的表达是异质性的。因此，在妊娠16天后，一些细胞是Lyt-1+2−，另一些是Lyt-1−2+，还有一些是Lyt-1+2+。然而，由于所讨论的各种原因，我们不愿认为这一发现证明了各种T细胞亚群的独立派生。
The appearance of cell membrane antigens on lymphoid cells of the embryonic thymus in vivo and in vitro using monoclonal antibodies in immunoperoxidase tests on frozen sections and in two‐color immunofluorescence tests on cell suspensions was studied. It was shown that cells are heterogeneous with respect to Lyt antigen expression from the earliest stages of ontogeny. Thus, after 16 days gestation, some cells are Lyt‐1+2−, others are Lyt‐1−2+ and some are Lyt‐1+2+. However, for various reasons which are discussed, we hesitate to suggest that this finding demonstrates independent derivation of the various T cell subsets.