A droplet-based microfluidic device for long-term culture and longitudinal observation of Caenorhabditis elegans
A droplet-based microfluidic device for long-term culture and longitudinal observation of Caenorhabditis elegans
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一种基于液滴的微流体装置，用于秀丽隐杆线虫的长期培养和纵向观察
DOI：
10.1007/s13206-012-6301-6
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发表时间：
2012-09-20
期刊：
影响因子：
4.3
通讯作者：
Luo, Guoan
中科院分区：
文献类型：
--
作者：
Ying, Dawei;Zhang, Kai;Luo, Guoan
介绍了一种基于液滴的微流控装置，用于秀丽隐杆线虫的长期培养和纵向观察。将线虫包裹在油包水液滴中，并可控地引入单独的腔室，在腔室中可通过重力驱动技术操纵培养基/油或培养基/空气界面，以进行终身培养。该装置还包含用于分别可逆地固定每条线虫的夹具结构，因此我们能够追踪每条线虫的更多细节。对线虫从第四幼虫期到死亡的几种表型进行了监测。然后利用该装置研究了线虫体长、摆动频率和寿命之间的关系。该装置在秀丽隐杆线虫的各种纵向研究中具有潜力，包括衰老、药物筛选、毒性评估等。
A droplet-based microfluidic device for long-term culture and longitudinal observation of Caenorhabditis elegans was presented. The worms were encapsulated in W/O droplets and controllably introduced into separate chambers, where the medium/oil or medium/air interface can be manipulated by a gravity-actuated technique, for lifelong culture. This device also contained clamp structures for reversibly immobilization of each worm separately, and thus we were able to track more details about each worm. Several phenotypes of worms were monitored from their fourth larva stage to death. The relationship between worm body length, stroke frequency and lifespan with this device was then studied. This device had a potential in various longitudinal researches on C. elegans including aging, drug screening, toxicity evaluation and etc.