Complete Mitochondrial Genome Sequence of the Tyrolean Iceman
Complete Mitochondrial Genome Sequence of the Tyrolean Iceman
复制标题
DOI：
10.1016/j.cub.2008.09.028
复制
发表时间：
2008-11-11
期刊：
影响因子：
9.2
通讯作者：
Rollo, Franco
中科院分区：
文献类型：
--
作者：
Ermini, Luca;Olivieri, Cristina;Rollo, Franco
蒂罗尔冰人是5350-5100年前中欧新石器时代-铜器时代转变的见证者，他的木乃伊尸体于1991年在奥地利和意大利边境的阿尔卑斯山冰川中被发现[1]。使用混合测序程序的基础上PCR扩增和454测序的合并扩增产物，我们已经检索到第一个完整的史前欧洲人的骨骼基因组序列。然后，我们将其与线粒体单倍群K的115个相关的现存谱系进行了比较。我们发现冰人属于线粒体单倍群K1的一个分支，在现代欧洲人群中还没有发现。这是迄今为止最古老的完整的智人mtDNA基因组。这些结果指出了完整的古代mtDNA研究在解决现代谱系的遗传地理学无法解决的人类种群遗传史问题方面的潜在意义。
The Tyrolean Iceman was a witness to the Neolithic-Copper Age transition in Central Europe 5350-5100 years ago, and his mummified corpse was recovered from an Alpine glacier on the Austro-Italian border in 1991 [1]. Using a mixed sequencing procedure based on PCR amplification and 454 sequencing of pooled amplification products, we have retrieved the first complete mitochondrial-genome sequence of a prehistoric European. We have then compared it with 115 related extant lineages from mitochondrial haplogroup K. We found that the Iceman belonged to a branch of mitochondrial haplogroup K1 that has not yet been identified in modern European populations. This is the oldest complete Homo sapiens mtDNA genome generated to date. The results point to the potential significance of complete-ancient-mtDNA studies in addressing questions concerning the genetic history of human populations that the phylo-geography of modern lineages is unable to tackle.