Identification of Bloodmeals in Wild Caught Blood Fed Phlebotomus argentipes (Diptera: Psychodidae) Using Cytochrome b PCR and Reverse Line Blotting in Bihar, India
Identification of Bloodmeals in Wild Caught Blood Fed Phlebotomus argentipes (Diptera: Psychodidae) Using Cytochrome b PCR and Reverse Line Blotting in Bihar, India
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DOI：
10.1603/me11115
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发表时间：
2012-05-01
影响因子：
2.1
通讯作者：
Poche, Richard
中科院分区：
文献类型：
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作者：
Garlapati, Rajesh B.;Abbasi, Ibrahim;Poche, Richard
通过确定自然栖息地中野生白蛉的宿主偏好，鉴定媒介中血粉的来源在流行病学研究中发挥着重要作用。亲人指数是人类利什曼病的重要组成部分。血粉分析可以识别媒介传播疾病的储存宿主。线粒体细胞色素 b 基因的扩增，以及随后的反向线印迹分析，有助于识别野生捕获的白蛉摄入的血粉。在当前的研究中，通过使用疾病控制中心的白炽灯迷你诱捕器，从印度比哈尔邦的三个不同村庄收集了吸血白蛉。陷阱被放置在村庄的五个不同地点。从喂血白蛉中提取全基因组 DNA，并扩增细胞色素 b 区域，然后进行反向线印迹分析。总共对 442 只吸血沙蝇进行了分析，其中 288 只 (65%) 细胞色素 b 聚合酶链反应呈阳性。人类、牛、水牛和山羊是确定的主要血粉，其次是鸡。在一些吸血白蛉中，发现了多种血粉。在目前的研究中，白蛉主要以人类为食，其次是牛、水牛和山羊。在这方面，在印度比哈尔邦进行病媒控制时，有必要同时考虑牛、水牛和山羊。
Identification of the source of bloodmeals in vectors plays an important role in epidemiological studies by determining the host preferences of wild sand flies in natural habitat. The anthropophilic index is a crucial component in human leishmaniasis. Bloodmeal analysis can identify the reservoir hosts of vector borne diseases. The amplification of the mitochondrial cytochrome b gene, followed by reverse line blot analysis, helps to identify the bloodmeal ingested by the wild caught sand flies. In the current study, blood fed sand flies were collected from three different villages in Bihar, India, by using Centers for Disease Control mini traps with incandescent light. Traps were placed in five different sites in the villages. Whole genome DNA was extracted from the blood fed sand flies and was amplified for the cytochrome b region, followed by reverse line blot analysis. In total, 442 blood fed sand flies were analyzed out of which 288 (65%) were positive to cytochrome b polymerase chain reaction. Humans, cattle, buffalo, and goats were the major bloodmeals identified, followed by chickens. In some of the blood fed sand flies, multiple bloodmeals were identified. In the current study, sand flies mostly fed on humans, followed by cattle, buffalo, and goats. In this regard, it is necessary to also consider cattle, buffalo, and goats when addressing vector control in Bihar, India.