Protein content of human apatite and brushite kidney stones: significant correlation with morphologic measures.
Protein content of human apatite and brushite kidney stones: significant correlation with morphologic measures.
复制标题
DOI：
10.1007/s00240-008-0151-7
复制
发表时间：
2008-10
影响因子：
--
通讯作者：
Williams, James C., Jr.
中科院分区：
文献类型：
--
作者：
Pramanik, Rocky;Asplin, John R.;Jackson, Molly E.;Williams, James C., Jr.
Apatite and brushite kidney stones share calcium and phosphate as their main inorganic components. We tested the hypothesis that these stone types differ in the organic matrix present in the stones. Intact stones were intensively analyzed by micro-computed tomography (micro CT) for both morphology (including the volume of voids, i.e., regions without mineral) and mineral type. In order to extract all proteins present in kidney stones in soluble form we developed a three-step extraction procedure using the ground stone powder. Apatite stones had significantly higher levels of total protein content and void volume compared to brushite stones. The void volume was highly correlated with the total protein contents in all stones (r2=0.61, P<0.0001), and brushite stones contained significantly fewer void regions and proteins than did apatite stones (3.2±4.5% voids for brushite vs. 10.8±11.2% for apatite, P<0.005; 4.1±1.6% protein for brushite vs. 6.0±2.4% for apatite, P<0.03). Morphological observations other than void volume did not correlate with protein content of stones, and neither did the presence or absence of minor mineral components. Our results show that protein content of brushite and apatite stones is higher than was previously thought, and also suggest that micro CT-visible void regions are related to the presence of protein.
登录
查看更多内容
影响因子：
5
作者：
Mushtaq, Shamim;Siddiqui, Anwar Ali;Shafqat, Jawed
通讯作者：
Shafqat, Jawed
影响因子：
19.6
作者：
Parks, JH;Worcester, EM;Lingeman, JE
通讯作者：
Lingeman, JE
影响因子：
5.9
作者：
BOYCE, WH
通讯作者：
BOYCE, WH
影响因子：
2.9
作者：
Kaneko, K;Yamanobe, T;Fujimori, S
通讯作者：
Fujimori, S
影响因子：
6.6
作者：
HERRING, LC
通讯作者：
HERRING, LC