The comparison of commercially available β-galactosidases for dairy industry : review
The comparison of commercially available β-galactosidases for dairy industry : review
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用于乳品工业的市售β-半乳糖苷酶的比较：综述
DOI：
10.22616/rrd.23.2017.032
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
I. Ciproviča
中科院分区：
文献类型：
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作者：
K. Žolnere;I. Ciproviča
β-半乳糖苷酶（EC 3.2.1.23）是广泛用于无乳糖乳生产和乳清渗透物处理的酶之一。酶可以从微生物、植物和动物中获得。如今，微生物正成为生产商业上可获得的酶的重要来源，这些酶受到极大关注并且提供了几个优点，例如容易处理和高产量。本文综述了β-半乳糖苷酶水解乳糖的研究进展，分析比较了β-半乳糖苷酶水解乳糖的最佳酶制剂。结果表明，选择合适的β-半乳糖苷酶水解乳糖的主要因素是反应条件。来自微生物的酶含有从4.0（简青霉和尼日尔曲霉）到8.5（枯草芽孢杆菌）的宽范围的最佳pH。最具商业潜力的酶是从真菌（黑曲霉和尼日尔）和酵母（乳酸克鲁维酵母和脆壁克鲁维酵母）中获得的酶。真菌来源的酶更适合于水解酸性乳清中的乳糖，因为其酸性pH，而酵母来源的酶用于牛奶和甜乳清。在这项研究中，从不同的供应商的商业水解物的目的，分析其乳糖水解潜力，并给出更详细的特点，每个水解物的优点和缺点也进行了总结。
β-Galactosidase (EC 3.2.1.23) is one of the widely used enzymes for lactose-free milk production and whey permeate treatment. Enzymes can be obtained from microorganisms, plants and animals. Nowadays, microorganisms are becoming an important source for production of commercially available enzymes, which are of great interest and offer several advantages such as easy handling and high production yield. The aim of this review was to summarize findings of research articles on the application of commercially available β-galactosidase preparates in dairy industry, to analyse and compare the most suitable β-galactosidase commercial preparates for lactose hydrolysis. The results showed that the main factor to choose an appropriate β-galactosidase for lactose hydrolysis was reaction condition. Enzymes from microorganisms contain a wide range of optimal pH from 4.0 (Penicillium simplicissimum and Aspergillus niger) to 8.5 (Bacillus subtilis). The greatest commercial potential have enzymes obtained from fungi (Aspergillus oryzae and Aspergillus niger) and yeasts (Kluyveromyces lactis and Kluyveromyces fragilis). Fungal origin enzymes are more suitable for the hydrolysis of lactose in acid whey due to its acidic pH but yeasts origin enzymes for milk and sweet whey. In the study, commercial preparates from different suppliers with the purpose to analyse their lactose hydrolysis potential and give more detailed characteristics of each preparate advantages and drawbacks were also summarized.