Roles of CO2, O2, and acid in arteriovenous [H+] difference during muscle contractions.
Roles of CO2, O2, and acid in arteriovenous [H+] difference during muscle contractions.
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CO2、O2 和酸在肌肉收缩期间动静脉 [H ] 差异中的作用。
DOI：
10.1152/jappl.1988.65.4.1803
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Eitzman,PD
中科院分区：
文献类型：
--
作者：
Stainsby,WN;Eitzman,PD
为了确定收缩期间肌肉动静脉[H+]差异的来源，在麻醉犬腓肠肌-跖肌群进行4/s等长收缩0.5、5.0和30.0 min之前和之后采集动脉和肌肉静脉血样对。分析这些样本的PO 2、PCO 2和pH值，全血中O2、CO2、K+、Na+、La-和Cl-的浓度，以及血浆中La-、K+、Na+和Cl-的浓度。对全血进行溶血，并分析PO 2、PCO 2和pH值。除非CO2酸（HA）和强离子差（[SID]）和常见离子[SID]（[K+] + [Na+] - [Cl-] - [La-]）的净输出外，还计算净O2摄取量、CO2输出量、L、K+、Na+和Cl-。根据这些数据，我们通过常见的PCO 2-pH图和[H+]-PCO 2图划分了动静脉[H+]差异的来源，并确定了真实的血浆动静脉[H+]差异是否反映了血浆和细胞动静脉[H+]差异。血浆和溶血血液的动静脉[H+]差异相同，表明真实血浆确实反映了血浆和细胞。K+表现出小的显著但短暂的输出。Na+不显著，而Cl-表现出显著的瞬时摄取。根据犬血液酸碱度计算的乳酸盐输出量和HA显示一过性输出量，并且相同。在5.0分钟时，动静脉差异最大，单独CO2将使[H+]增加15.9 nmol/l，而Hb去饱和将使[H+]减少4.2 nmol/l，乳酸盐可使[H+]增加1.0 nmol/l。（250字处删节）
To determine the origins of the arteriovenous [H+] difference of muscle during contractions, arterial and muscle venous blood sample pairs were taken before and after 0.5, 5.0, and 30.0 min of 4/s isometric twitches of the gastrocnemius-plantaris muscle group of anesthetized dogs. These samples were analyzed for PO2, PCO2, and pH, the concentrations of O2, CO2, K+, Na+, La-, and Cl- in whole blood, and La-, K+, Na+, and Cl- in plasma. Whole blood was hemolyzed and analyzed for PO2, PCO2, and pH. Net O2 uptake, CO2 output, L, K+, Na+, and Cl- were calculated in addition to net output of non-CO2 acid (HA) and strong ion difference ([SID]) and common ion [SID] ([K+] + [Na+] - [Cl-] - [La-]). From these data we partitioned the origins of the arteriovenous [H+] difference via the common PCO2-pH diagram and via a [H+]-PCO2 diagram and determined whether true plasma arteriovenous [H+] differences reflect plasma and cell arteriovenous [H+] differences. The arteriovenous [H+] differences of plasma and hemolyzed blood were the same, showing that true plasma does reflect plasma and cells. K+ showed a small significant but transient output. Na+ was not significant, whereas Cl- showed a significant transient uptake. Lactate output and HA, calculated for dog blood acid-base, showed transient outputs and were the same. At 5.0 min when the arteriovenous difference was largest, CO2 alone would have increased [H+] 15.9 nmol/l whereas desaturation of Hb would have decreased [H+] 4.2 nmol/l and lactate could have raised [H+] 1.0 nmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)