Carcinoma-specific Ulex europaeus agglutinin-I binding glycoproteins of human colorectal carcinoma and its relation to carcinoembryonic antigen.
Carcinoma-specific Ulex europaeus agglutinin-I binding glycoproteins of human colorectal carcinoma and its relation to carcinoembryonic antigen.
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人结直肠癌癌特异性欧洲榆凝集素-I结合糖蛋白及其与癌胚抗原的关系。
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发表时间：
1985
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通讯作者：
E. Sato
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作者：
Y. Matsushita;S. Yonezawa;T. Nakamura;S. Shimizu;M. Ozawa;T. Muramatsu;E. Sato
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法和免疫印迹法对18例结直肠癌组织中识别α-L岩藻糖末端残基的凝集素结合糖蛋白进行了分析。在远端大肠(降结肠和乙状结肠和直肠)，癌组织中存在与UEA-I结合的大分子糖蛋白，而正常粘膜中不存在。在大肠近端(升结肠和横结肠)，与UEA-I结合的大分子糖蛋白在正常粘膜和癌组织中均有表达，而癌组织的UEA-I结合蛋白的相对分子质量明显低于正常粘膜。因此，UEA-I结合糖蛋白在正常大肠粘膜和癌组织之间存在生化差异，而在我们先前的组织化学研究中，癌组织和正常黏膜之间的大肠近端UEA-I结合糖蛋白没有差异。此外，来自癌组织的癌胚抗原被发现具有与UEA-I结合糖蛋白相同的电泳率。
Glycoproteins binding to Ulex europaeus agglutinin-I (UEA-I) lectin, which recognizes the terminal alpha-L-fucose residue, were analyzed in 18 cases of human colorectal carcinoma by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by the Western blotting method. In the distal large bowel (descending and sigmoid colon and rectum), high-molecular-weight glycoproteins binding to UEA-I existed in carcinoma tissue but not in normal mucosa. In the proximal large bowel (ascending and transverse colon), high-molecular-weight glycoproteins binding to UEA-I were found both in normal mucosa and in carcinoma tissue, whereas those from the carcinoma tissue had an apparently lower molecular weight as compared to the weight of those from the normal mucosa. Thus there is a biochemical difference in UEA-I binding glycoproteins between the normal mucosa and the carcinoma tissue, although in our previous histochemical study no difference was observed in UEA-I binding glycoproteins of the proximal large bowel between the carcinoma tissue and the normal mucosa. Furthermore, carcinoembryonic antigen from the carcinoma tissue was found to have the same electrophoretical mobility as the UEA-I binding glycoproteins.