THE COLORIMETRIC ESTIMATION OF FORMALDEHYDE BY MEANS OF THE HANTZSCH REACTION
THE COLORIMETRIC ESTIMATION OF FORMALDEHYDE BY MEANS OF THE HANTZSCH REACTION
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DOI：
10.1042/bj0550416
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发表时间：
1953-01-01
影响因子：
4.1
通讯作者：
NASH, T
中科院分区：
文献类型：
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作者：
NASH, T
试剂溶液。由乙酸铵 (2[图像])、乙酸 (0.05[图像]) 和乙酰丙酮 (0.02[图像]) 组成。将其与等体积混合。溶液。含量不超过8毫克。甲醛并静置后，在具有深蓝色滤光片的光电比色计中估计黄色(由于二乙酰二氢二甲基吡啶，DDL)(最大吸收在412mμ)。 5 小时内反应完成 99%。 20°，40 分钟。 37°，5 分钟。 58[度]）。该反应也可用于甲醛的检测，但应意识到该试剂的组分具有挥发性，并且DDL晶体在弱紫外光下具有强荧光。相同浓度的乙醛产生约1%的干扰。丙酮、三氯乙醛、糠醛和葡萄糖不干扰，胺类干扰轻微，高碘酸盐、硫酸盐、强氧化剂和日光会破坏DDL。
The reagent soln. consists of ammonium acetate (2[image]), acetic acid (0.05[image]) and acetylacetone (0.02[image]). This is mixed with an equal vol. of soln. containing not more than 8 mg. of formaldehyde and after standing, the yellow color (due to diacetyldihydrolutidine, DDL) is estimated in a photoelectric colorimeter with deep-blue filter (absorption max. at 412 m[mu]). Reaction is 99% complete in 5 hrs. at 20[degree], 40 mins. at 37[degree], and 5 mins. at 58[degree]). The reaction can also be used for the detection of formaldehyde but it should be realized that the components of the reagent are volatile and that DDL crystals are strongly fluorescent under soft u.-v. Acetaldehyde in the same concn. gives about 1% interference. Acetone, chloral, fural and glucose do not interfere, amines interfere slightly and periodate, sulfate, strong oxidizing agents and daylight destroy DDL.