Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells
Silver nanoparticle induced cytotoxicity, oxidative stress, and DNA damage in CHO cells
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DOI：
10.1007/s11051-013-1898-5
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发表时间：
2013-09-01
影响因子：
2.5
通讯作者：
John, P. J.
中科院分区：
文献类型：
--
作者：
Awasthi, Kumud Kant;Awasthi, Anjali;John, P. J.
银纳米颗粒由于具有抗菌活性，在伤口敷料、导尿管和各种家居用品中的应用越来越广泛。本文报道了利用中国仓鼠卵巢(CHO)细胞对合成的、具有良好特性的银纳米粒的毒性评价。UV-Vis光谱表明，在408-410 nm处出现典型的表面等离子体吸收峰，从而形成了银纳米粒子。透射电子显微镜显示，银纳米粒子的平均直径约为5.0+/-1.0 nm，呈球形。细胞能见度和细胞存活率显示银纳米粒的细胞毒性呈剂量依赖性。纳米银作用24 h，对CHO细胞的半数抑制浓度(IC50)为68.0+/-2.65微克/毫升。对未暴露的CHO细胞(对照组)和暴露于浓度为15、30和60 mU g/ml的Ag NPs作用24 h的CHO细胞进行了细胞形态、线粒体功能(四甲基偶氮唑盐比色法)、活性氧(ROS)和DNA片段化分析(梯形图谱)等毒性评价，为设计不同用途的Ag NPs提供了依据。
Silver nanoparticles (Ag NPs) are being used increasingly in wound dressings, catheters, and in various household products due to their antimicrobial activity. The present study reports the toxicity evaluation of synthesized and well characterized Ag NPs using Chinese hamster ovary (CHO) cells. The UV-Vis spectroscopy reveals the formation of silver nanoparticles by exhibiting the typical surface plasmon absorption maxima at 408-410 nm. Transmission electron microscopy (TEM) reveals that the average diameter of silver nanoparticles is about 5.0 +/- 1.0 nm and that they have spherical shape. Cell visibility and cell viability percentage show dose-dependent cellular toxicity of Ag NPs. The half maximal inhibitory concentration (IC50) for CHO cells is 68.0 +/- 2.65 mu g/ml after 24 h Ag NPs exposure. Toxicity evaluations, including cellular morphology, mitochondrial function (MTT assay), reactive oxygen species (ROS), and DNA fragmentation assay (Ladder pattern) were assessed in unexposed CHO cells (control) and the cells exposed to Ag NPs concentrations of 15, 30, and 60 mu g/ml for 24 h. The findings may assist in the designing of Ag NPs for various applications and provide insights into their toxicity.