Gated magnetic resonance imaging of normal and hypertrophied murine hearts.
Gated magnetic resonance imaging of normal and hypertrophied murine hearts.
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正常和肥大小鼠心脏的门控磁共振成像。
DOI：
10.1152/ajpheart.1997.272.5.h2394
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Gardin,JM
中科院分区：
文献类型：
--
作者：
Siri,FM;Jelicks,LA;Leinwand,LA;Gardin,JM
转基因小鼠模型正越来越多地用于探索心脏病的分子基础。直到最近，由于其非常小的尺寸和高心率，没有用于非侵入性评估小鼠心脏的质量和功能变化的方法。经胸超声心动图现在已被用来获得无创估计小鼠左心室（LV）壁厚，内部尺寸和质量。然而，这种方法是基于左心室中壁的一维（M模式）测量，没有考虑左心室腔和壁尺寸沿沿着其他短轴和其他解剖水平的变化。因此，可能影响LV质量估计的LV几何结构的不对称性可能未被检测到。在这项研究中，门控（舒张）磁共振成像（MRI）被用来获得二维图像的LV在四个解剖水平在完整的，麻醉小鼠。在17只正常CD-1小鼠（体重，18-47 g;重量LV质量，51-135 mg）中，由MRI数据产生的LV质量估计值与重量测定的LV质量相关性良好（r = 0.87）。此外，该方法确定了一组7只患有代偿性和失代偿性LV肥大的心脏收缩小鼠（体重，32-39 g; LV重量，119-198 mg）的LV质量和壁厚-腔室直径比的变化。这些结果表明，效用的MRI系列，非侵入性评估实验诱导的小鼠心脏的质量和几何形状的改变。
Transgenic murine models are being used increasingly to explore the molecular basis of heart disease. Until recently, there were no means for noninvasive assessment of changes in mass and function of the murine heart because of its very small size and high heart rate. Transthoracic echocardiography has now been utilized to obtain noninvasive estimates of murine left ventricular (LV) wall thicknesses, internal dimension, and mass. However, this approach is based on one-dimensional (M-mode) measurements of the LV at its midwall that take no account of variations in LV chamber and wall dimensions along other minor axes and at other anatomic levels. Thus asymmetries in LV geometry, which can affect LV mass estimates, may be undetected. In this study, gated (diastolic) magnetic resonance imaging (MRI) was utilized to obtain two-dimensional images of the LV at four anatomic levels in intact, anesthetized mice. In 17 normal CD-1 mice (body mass, 18-47 g; gravimetric LV mass, 51-135 mg), LV mass estimates produced from the MRI data correlated well (r = 0.87) with LV mass determined gravimetrically. In addition, this approach identified changes in LV mass and wall thickness-to-chamber diameter ratio in a group of seven aortic-constricted mice (body mass, 32-39 g; gravimetric LV mass, 119-198 mg) with compensated and decompensated LV hypertrophy. These findings suggest that utility of MRI for serial, noninvasive assessment of experimentally induced alterations in mass and geometry of the murine heart.