Yeast, plants, worms, and flies use a methyltransferase to metabolize age-damaged (R,S)-AdoMet, but what do mammals do?
Yeast, plants, worms, and flies use a methyltransferase to metabolize age-damaged (R,S)-AdoMet, but what do mammals do?
复制标题
酵母、植物、蠕虫和苍蝇使用甲基转移酶来代谢因年龄受损的 (R,S)-AdoMet，但哺乳动物会做什么呢？
DOI：
10.1089/rej.2009.0956
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发表时间：
2010
影响因子：
2.6
通讯作者：
Clarke,StevenG
中科院分区：
文献类型：
--
作者：
Vinci,ChrisR;Clarke,StevenG
生物甲基供体S-腺苷-L-甲硫氨酸[（S，S）-甲硫氨酸]在生理条件下可自发分解形成无活性的非对映体（R，S）-甲硫氨酸，其可干扰细胞功能。尽管一些低等生物通过同型半胱氨酸甲基转移酶代谢（R，S）-蛋氨酸，但哺乳动物如何处理它还不清楚。在本文中，我们证明了小鼠肝脏提取物，含有具有类似活性的BHMT-2同型半胱氨酸甲基转移酶候选物，识别（S，S）-蛋氨酸，但不识别（R，S）-蛋氨酸。我们没有发现这些提取物中（R，S）-蛋氨酸酶促分解的证据。因此，哺乳动物可以使用与其他生物体不同的策略代谢（R，S）-蛋氨酸。
The biological methyl donorS-adenosyl-l-methionine [(S,S)-AdoMet] can spontaneously break down under physiological conditions to form the inactive diastereomer (R,S)-AdoMet, which may interfere with cell function. Although several lower organisms metabolize (R,S)-AdoMet via homocysteine methyltransferases, it is unclear how mammals deal with it. In this paper, we show that the mouse liver extracts, containing the BHMT-2 homocysteine methyltransferase candidate for a similar activity, recognizes (S,S)-AdoMet but not (R,S)-AdoMet. We find no evidence for the enzymatic breakdown of (R,S)-AdoMet in these extracts. Thus, mammals may metabolize (R,S)-AdoMet using a different strategy than other organisms.