Increase in CD5L expression in the synovial membrane of knee osteoarthritis patients with obesity.
Increase in CD5L expression in the synovial membrane of knee osteoarthritis patients with obesity.
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DOI：
10.5114/ceji.2021.108180
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Takaso M
中科院分区：
文献类型：
--
作者：
Shoji S;Uchida K;Inoue G;Takata K;Mukai M;Aikawa J;Iwase D;Takano S;Sekiguchi H;Takaso M
肥胖似乎是膝骨性关节炎(KOA)发展的一个强大的危险因素，但其机制尚不完全清楚。CD5L在组织巨噬细胞中表达，在肥胖小鼠中表达增加。我们假设在肥胖的膝关节骨性关节炎患者的滑膜(SM)中CD5L的表达增加。在这里，我们研究了CD5L在这些患者SM中的表达。90例膝关节骨性关节炎患者(男26例，女)按世界卫生组织体重指数分类标准分为正常体质量组(NW，25 kg/m<sup>2)、超重组(OW，25~29.99 kg/m<sup>2)和肥胖组(≥，30 kg/m<sup>2</sup>)。用实时定量聚合酶链式反应比较各组SM中CD5L的表达。从SM中分离出CD14+(巨噬细胞组分)和CD14-(成纤维细胞组分)细胞，观察SM中CD5L的表达情况。CD5L在OB组的表达明显高于NW组和OW组(p<；0.001)。CD14+组分有CD5L表达，CD14-组分无CD5L表达。CD5L在肥胖的膝关节骨性关节炎患者的SM中高表达。CD5L在膝关节骨性关节炎病理与肥胖关系中的作用尚需进一步研究。
Obesity appears to be a powerful risk factor for the development of knee osteoarthritis (KOA), but the mechanisms of this are not fully understood. CD5L is expressed in tissue macrophages and is increased in obese mice. We hypothesized that CD5L expression is increased in the synovial membrane (SM) of obese KOA patients. Here, we investigated CD5L expression in the SM of these patients. Ninety KOA patients (26 males, 64 females) were allocated to one of three groups based on body mass index (BMI): normal weight (NW, < 25 kg/m<sup>2</sup>), overweight (OW, 25-29.99 kg/m<sup>2</sup>) and obese (OB, ≥ 30 kg/m<sup>2</sup>), according to the World Health Organization BMI classification (each n = 30). Expression of CD5L in SM among the groups was compared using real-time polymerase chain reaction. To investigate CD5L-expressing cells in SM, CD14+ (macrophage fraction) and CD14– (fibroblast fraction) cells were separated from the SM. CD5L expression was significantly higher in the OB group than in the NW and OW groups (p < 0.001). CD5L expression was observed in the CD14+ fraction but not in the CD14– fraction. CD5L is highly expressed in the SM of KOA patients with obesity. Further investigation is required to identify the role of CD5L in the relationship between KOA pathology and obesity.
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影响因子：
4.1
作者：
Takano S;Uchida K;Miyagi M;Inoue G;Fujimaki H;Aikawa J;Iwase D;Minatani A;Iwabuchi K;Takaso M
通讯作者：
Takaso M
影响因子：
4.6
作者：
Pearson MJ;Herndler-Brandstetter D;Tariq MA;Nicholson TA;Philp AM;Smith HL;Davis ET;Jones SW;Lord JM
通讯作者：
Lord JM
影响因子：
3.7
作者：
Sun AR;Panchal SK;Friis T;Sekar S;Crawford R;Brown L;Xiao Y;Prasadam I
通讯作者：
Prasadam I
影响因子：
2.7
作者：
Kim, Hee Nam;Kim, Yeo-Kyeoung;Kim, Hyeoung-Joon
通讯作者：
Kim, Hyeoung-Joon
影响因子：
3.8
作者：
Gray J;Chattopadhyay D;Beale GS;Patman GL;Miele L;King BP;Stewart S;Hudson M;Day CP;Manas DM;Reeves HL
通讯作者：
Reeves HL