Quantification of polymer interactions in bacterial adhesion
Quantification of polymer interactions in bacterial adhesion
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DOI：
10.1021/es980211s
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发表时间：
1998-10-01
影响因子：
11.4
通讯作者：
Harms, H
中科院分区：
文献类型：
--
作者：
Jucker, BA;Zehnder, AJB;Harms, H
细菌对固体的粘附受到范德华力、静电和酸碱（疏水）相互作用的控制，这些相互作用在扩展 DLVO 模型 (DLVO-AB) 中结合起来，并受到细菌表面聚合物与固体表面的相互作用的控制。目前还没有计算聚合物相互作用的方法，并且只能根据实际粘附力与基于 DLVO-AB 的预期的偏差定性地推断出它们的存在。在这里，我们尝试（i）根据这种偏差量化聚合物相互作用，以及（ii）独立计算它们作为排斥和吸引贡献的总和。假定排斥力是由聚合物层对压缩的抵抗力产生的。其计算对细胞膜中聚合物的堆积密度最敏感。假设吸引力源自聚合物对表面的吸附，并根据分离的表面聚合物的吸附数据进行计算。六种革兰氏阴性细菌与玻璃粘附的总相互作用能曲线的比较表明，五种菌株的低粘附可能是由于占主导地位的聚合物排斥造成的，即难以压缩的细胞包膜阻碍了细菌接近DLVO-AB相互作用产生的能量最小值。一种应变很容易粘附，可能是因为聚合物排斥力较低，而聚合物吸引力和 DLVO-AB 力主导了整体相互作用。
Adhesion of bacteria to solids is governed by van der Waals, electrostatic, and acid-base (hydrophobic) interactions, which are combined in an extended DLVO model (DLVO-AB) and by interactions of bacterial surface polymers with the solid surfaces. A method to calculate polymer interactions was not available yet, and their existence had been inferred only qualitatively from the deviation of the actual adhesion from DLVO-AB-based expectations. Here, we present attempts (i) to quantify polymer interactions from this deviation and (ii) to calculate them independently as the sum of repulsive and attractive contributions. Repulsion was assumed to result from the resistance of the polymer layer against compression. Its calculation was most sensitive to the packing density of the polymers in the cell envelope. Attraction was assumed to origin from polymer adsorption to the surface and was calculated on the basis of adsorption data of isolated surface polymers. Comparison of total interaction energy curves with adhesion of six Gram-negative bacteria to glass showed that the low adherence of five strains may have resulted from dominant polymer repulsion, i.e., hardly compressible cell envelopes hindered the bacteria to approach the energy minima resulting from DLVO-AB interactions. One strain adhered readily, possibly because polymer repulsion was low and polymer attraction and DLVO-AB forces dominated the overall interaction.