Sensitivity and specificity of in situ bacterial chain reaction (BCR) in detecting sparse human tumor cells in peripheral blood.
Sensitivity and specificity of in situ bacterial chain reaction (BCR) in detecting sparse human tumor cells in peripheral blood.
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原位细菌链式反应（BCR）检测外周血中稀疏人类肿瘤细胞的敏感性和特异性。
DOI：
10.1006/bbrc.1996.1760
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Craig,AJ
中科院分区：
文献类型：
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作者：
Rotman,B;Guzman,R;Craig,AJ
我们在这里报告细菌链反应（BCR）的发展，一个系统使用微生物作为纳米器件来放大和可视化来自分子生物探针（如抗体，结合蛋白，凝集素和寡核苷酸）的信号。与酶量为恒定参数的常规酶联扩增系统不同，在BCR中，酶（青霉素酶）用于触发增殖链反应，产生酶的指数增加。使用设计用于检测和计数以极低频率（例如，每百万个正常细胞中有一个肿瘤细胞）。对83例健康献血员外周血标本的检测结果表明，特异性为97.6%。该系统能够以2 × 10−7的频率检测肿瘤细胞。
We report here the development of bacterial chain reaction (BCR), a system using micro organisms as nanodevices to amplify and visualize signals from molecular bioprobes such as antibodies, binding proteins, lectins, and oligonucleotides. Unlike conventional enzyme-linked amplification systems in which the amount of enzyme is a constant parameter, in the BCR an enzyme (penicillinase) is used to trigger a proliferative chain reaction producing an exponential increase in enzyme. The detection limits and specificity of BCR were determined using a model system designed to detect and enumerate MCF-7 (a human breast adenocarcinoma cell line) cells disseminated at extremely low frequency (e.g., one tumor cell per million normal cells) among monocluclear cells (MNCs) of human peripheral blood. Results of testing 83 specimens of peripheral blood from presumably healthy donors showed 97.6% specificity. The system was capable of detecting tumor cells at a frequency of 2 × 10−7.