Linkage mapping of 1454 new maize candidate gene loci
Linkage mapping of 1454 new maize candidate gene loci
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DOI：
10.1534/genetics.104.040204
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发表时间：
2005-08-01
期刊：
影响因子：
3.3
通讯作者：
Murigneux, A
中科院分区：
文献类型：
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作者：
Falque, M;Décousset, L;Murigneux, A
对玉米EST序列的生物信息学分析已经突出了大量与农业重要性状有关的候选基因。为了有助于正在进行的这些基因的定位工作，我们使用了两个交配重组自交系(IRIL)群体，它们允许比非交配重组自交系更高的图谱分辨率。第一个面板(IBM)源自B73 X Mo17，可从玉米遗传合作库存中心公开购买。第二个面板(LHRF)是从F2×F252发展而来的，用于在IBM上定位单态基因座。我们从IBM面板构建了237个基因座的框架图，从LHRF面板构建了271个基因座的框架图。这两个图谱被用来定位1454个位点(1056个位于IBM_Gnp2004上，398个位于LHRF_Gnp2004上)，对应于954个先前未定位的cDNA探针。目前主要使用的是RFLP，但也有基于聚合酶链式反应的方法对部分cDNA进行SNP定位。与迄今发布的基于IRIL的常规图谱不同，修正后的减数分裂厘米级距离是考虑到IRIL经历的交配世代数而计算出来的。我们的框架图的校正大小分别为IBM_Gnp2004的1825 cM和LHRF_Gnp2004的1862 cM。在LHRF_Gnp2004上定位的所有基因座也被投影到一个共识图IBMConsensus_Gnp2004上。除I号和8号染色体外，其余基因座在着丝粒附近形成簇状。
Bioinformatic analyses of maize EST sequences have highlighted large numbers of candidate genes putatively involved in agriculturally important traits. To contribute to ongoing efforts toward mapping of these genes, we used two populations of intermated recombinant inbred lines (IRILs), which allow a higher map resolution than nonintermated RILs. The first panel (IBM), derived from B73 X Mo17, is publicly available from the Maize Genetics Cooperation Stock Center. The second panel (LHRF) was developed from F2 X F252 to map loci monomorphic on IBM. We built framework maps of 237 loci from the IBM panel and 271 loci from the LHRF panel. Both maps were used to place 1454 loci (1056 on map IBM_Gnp2004 and 398 on map LHRF_Gnp2004) that corresponded to 954 cDNA probes previously unmapped. RFLP was mostly used, but PCR-based methods were also performed for some cDNAs to map SNPs. Unlike in usual IRIL-based maps published so far, corrected meiotic centimorgan distances were calculated, taking into account the number of intermating generations undergone by the IRILs. The corrected sizes of our framework maps were 1825 cM for IBM_Gnp2004 and 1862 cM for LHRF_Gnp2004. All loci mapped on LHRF_Gnp2004 were also projected on a consensus map IBMconsensus_Gnp2004. cDNA loci formed clusters near the centromeres except for chromosomes I and 8.