Proximity-Driven DNA Nanosensors.
Proximity-Driven DNA Nanosensors.
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DOI：
10.1149/2754-2726/ace068
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发表时间：
2023-09-01
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在邻近驱动的传感中，探针和分析物之间的相互作用通过引起两个探针组分或信号传导部分的距离变化而产生可检测信号。通过将这样的系统与基于DNA的纳米结构连接，可以设计出高度灵敏、特异和可编程的平台。在这个角度来看，我们描绘了在邻近驱动的纳米传感器中使用DNA构建块的优势，并概述了该领域的最新进展，从快速检测食品中农药的传感器到识别血液中罕见癌细胞的探针。我们还讨论了当前的挑战，并确定了需要进一步发展的关键领域。邻近驱动的DNA纳米传感器（PDN）被广泛用于生物化学传感。离子强度、温度、pH和核酸酶可影响PDN的稳定性。靶向部分可用于将PDN引导至特定细胞和组织。需要进一步的研究来提高PDN的灵敏度和选择性。需要进一步研究将PDN转化为现场/即时护理设备。
In proximity-driven sensing, interactions between a probe and an analyte produce a detectable signal by causing a change in distance of two probe components or signaling moieties. By interfacing such systems with DNA-based nanostructures, platforms that are highly sensitive, specific, and programmable can be designed. In this Perspective, we delineate the advantages of using DNA building blocks in proximity-driven nanosensors and provide an overview of recent progress in the field, from sensors that rapidly detect pesticides in food to probes that identify rare cancer cells in blood. We also discuss current challenges and identify key areas that need further development. Proximity-driven DNA nanosensors (PDNs) are widely used in biochemical sensing. Ionic strength, temperature, pH, and nucleases can affect the stability of PDNs. Targeting moieties can be used to direct PDNs to specific cells and tissues. Further research is needed to improve the sensitivity and selectivity of PDNs. Further research is needed to translate PDNs into on-site/point-of-care devices.