MAPK inactivation is required for the G(2) to M-phase transition of the first mitotic cell cycle
MAPK inactivation is required for the G(2) to M-phase transition of the first mitotic cell cycle
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DOI：
10.1093/emboj/16.21.6407
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发表时间：
1997-11-03
期刊：
影响因子：
11.4
通讯作者：
Picard, A
中科院分区：
文献类型：
--
作者：
Abrieu, A;Fisher, D;Picard, A
MAP激酶（MAPK）的下调是动物界受精的普遍结果，尽管其作用尚不清楚。在这里，我们表明MAPK失活对于脊椎动物和无脊椎动物的胚胎进入第一次有丝分裂是必不可少的。在受精时抑制MAPK的下调，例如通过组成性激活上游MAPK级联，特异性抑制细胞周期蛋白B-cdc 2激酶的激活及其结果，进入第一次有丝分裂。因此，MAPK似乎通过阻止未受精卵进行孤雌发育而在减数分裂成熟中起作用。人工维持受精后MAPK活性的最普遍作用是阻止第一次有丝分裂细胞周期中G（2）期向M期的转变，尽管受精后MAPK的不适当再激活可能导致脊椎动物的中期阻滞，但提前受精卵中MAPK的失活时间并不能加速其进入第一次有丝分裂。因此，在第一次有丝分裂细胞周期的一部分持续的MAPK活性是不负责受精卵进入第一次有丝分裂的后期。
Down-regulation of MAP kinase (MAPK) is a universal consequence of fertilization in the animal kingdom, although its role is not known, Here we show that MAPK inactivation is essential for embryos, both vertebrate and invertebrate, to enter first mitosis, Suppressing down-regulation of MAPK at fertilization, for example by constitutively activating the upstream MAPK cascade, specifically suppresses cyclin B-cdc2 kinase activation and its consequence, entry into first mitosis, It thus appears that MAPK functions in meiotic maturation by preventing unfertilized eggs from proceeding into parthenogenetic development. The most general effect of artificially maintaining MAPK activity after fertilization is prevention of the G(2) to M-phase transition in the first mitotic cell cycle, even though inappropriate reactivation of MAPK after fertilization may lead to metaphase arrest in vertebrates, Advancing the time of MAPK inactivation in fertilized eggs does not, however, speed up their entry into first mitosis. Thus, sustained activity of MAPK during part of the first mitotic cell cycle is not responsible for late entry of fertilized eggs into first mitosis.