Simultaneous expression of regulatory genes associated with specific drought-adaptive traits improves drought adaptation in peanut
Simultaneous expression of regulatory genes associated with specific drought-adaptive traits improves drought adaptation in peanut
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DOI：
10.1111/pbi.12461
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发表时间：
2016-03-01
影响因子：
13.8
通讯作者：
Udayakumar, Makarla
中科院分区：
文献类型：
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作者：
Ramu, Vemanna S.;Swetha, Thavarekere N.;Udayakumar, Makarla
作物适应易干旱的雨水灌溉条件，可以通过改善植物性状，如有效的水开采（通过上级根系性状）和细胞水平的耐受机制来实现。通过调节干旱适应机制的基因的同时表达来聚合这些干旱适应性状在提高胁迫耐受性方面具有显著的相关性。在这项研究中，我们提供的证据表明，花生转基因植物表达er 1（Alfin 1），根生长相关的转录因子基因，狼尾草热休克因子（PgHSF 4）和豌豆DNA解旋酶（PDH 45）参与蛋白质周转和保护表现出更好的耐受性，更高的生长和生产力在干旱胁迫条件下。在转基因系中观察到所有转基因的稳定整合。转基因株系在干旱胁迫下表现出较高的根系生长量、较低的冠层空气温差（较小的CCATD）和较高的相对含水量（RWC）。低脯氨酸水平的转基因株系证实较高的水分状况的维护。在具有较高生物量的逐渐水分胁迫条件下，转基因株系的存活和恢复显著较高。转基因株系对乙烯利诱导的衰老和甲基紫精诱导的氧化胁迫也表现出明显的耐受性。几个应激反应基因，如热休克蛋白（HSP），环盒蛋白-1（RBX 1），醛糖还原酶，晚期胚胎发育丰富-5（LEA 5）和富含脯氨酸的蛋白-2（PRP 2），参与根生长的基因，在转基因株系中表现出增强的表达在胁迫下。因此，干旱适应性性状的调控基因的同时表达可以提高作物在水分有限条件下的适应性和生产力。
Adaptation of crops to drought-prone rain-fed conditions can be achieved by improving plant traits such as efficient water mining (by superior root characters) and cellular-level tolerance mechanisms. Pyramiding these drought-adaptive traits by simultaneous expression of genes regulating drought-adaptive mechanisms has phenomenal relevance in improving stress tolerance. In this study, we provide evidence that peanut transgenic plants expressing er 1 (Alfin1), a root growth-associated transcription factor gene, Pennisetum glaucum heat-shock factor (PgHSF4) and Pea DNA helicase (PDH45) involved in protein turnover and protection showed improved tolerance, higher growth and productivity under drought stress conditions. Stable integration of all the transgenes was noticed in transgenic lines. The transgenic lines showed higher root growth, cooler crop canopy air temperature difference (less CCATD) and higher relative water content (RWC) under drought stress. Low proline levels in transgenic lines substantiate the maintenance of higher water status. The survival and recovery of transgenic lines was significantly higher under gradual moisture stress conditions with higher biomass. Transgenic lines also showed significant tolerance to ethrel-induced senescence and methyl viologen-induced oxidative stress. Several stress-responsive genes such as heat-shock proteins (HSPs), RING box protein-1 (RBX1), Aldose reductase, late embryogenesis abundant-5 (LEA5) and proline-rich protein-2 (PRP2), a gene involved in root growth, showed enhanced expression under stress in transgenic lines. Thus, the simultaneous expression of regulatory genes contributing for drought-adaptive traits can improve crop adaptation and productivity under water-limited conditions.