Expression of c-kit protooncogene in human hepatocellular carcinoma
Expression of c-kit protooncogene in human hepatocellular carcinoma
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DOI：
10.1016/j.canlet.2004.06.045
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发表时间：
2005-01-20
期刊：
影响因子：
9.7
通讯作者：
Chang, CS
中科院分区：
文献类型：
--
作者：
Chung, CY;Yeh, KT;Chang, CS
研究c-kit原癌基因在人肝细胞癌中的表达。免疫组织化学(IHC)法检测c-kit原癌基因的蛋白表达，RT-PCR法检测c-kit原癌基因的mRNA表达。免疫组化结果显示，86例肝癌组织切片中有22例(25.6%)表达c-kit蛋白。RT-PCR进一步证实22例IHC c-kit阳性肝细胞癌组织中均有c-kit基因转录。此外，对c-kit在肝细胞癌中的表达与患者预后的关系进行了统计分析，建立了相关性。C-Kit染色阳性的肝癌患者与c-Kit表达阴性的患者相比存活率更高(p=0.021)。这些结果提示c-kit的表达可能是肝细胞癌良好的预后指标。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
The expression of the c-kit protooncogene in human hepatocellular carcinoma (HCC) was investigated. Immunohistochemical staining (IHC) and RT-PCR were employed to examine the protein and mRNA expression of c-kit protooncogene, respectively. IHC results demonstrated that 22 of 86 (25.6%) HCC tissue sections expressed c-kit protein. The c-kit mRNA transcript was further confirmed in all 22 IHC c-kit positive HCC tissue samples by RT-PCR. Moreover, the relationship between c-kit expression in HCC and prognosis of patients was statistically analyzed and a correlation was established. The group of patients whose HCC specimens showed positive c-kit staining exhibited better survival as compared to those patients with negative c-kit expression (p=0.021). These results suggest that c-kit expression may be a good prognostic indicator for HCC. (C) 2004 Elsevier Ireland Ltd. All rights reserved.