Carbon Sources in Riverine Food Webs: New Evidence from Amino Acid Isotope Techniques
Carbon Sources in Riverine Food Webs: New Evidence from Amino Acid Isotope Techniques
复制标题
河流食物网中的碳源：氨基酸同位素技术的新证据
DOI：
10.1007/s10021-016-0091-y
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
R. Bowes
中科院分区：
文献类型：
--
作者：
J. Thorp;R. Bowes
近40年来，关于生态系统中支持动物生产的碳的起源的争论已经产生了许多概念性的理论，强调了原生碳、陆地碳或两者(取决于河流水位高度)的重要性。由于缺乏足够的分析方法来区分消费者组织中的终极本土碳和异地碳，测试理论一直受到阻碍。研究人员最初依赖于肠道内容物的同化效率，后来又依赖于大宗组织稳定同位素分析或脂肪酸方法。然而，氨基酸的最新技术，化合物特定稳定同位素分析(AA-CSIA)，使调查人员能够通过追踪必需氨基酸从生产者到消费者，将消费者与食品来源联系起来。我们使用AA-CSIA对两条水文地貌不同的大河：密西西比河上游(UMR)和最狭窄的俄亥俄河下游(LOR)的5种食虫性物种和6种食鱼性物种的营养来源进行了评估。我们用同位素分析的博物馆标本几十年来一直被其他研究人员收集(UMR：1900-1969；LOR：1931-1970)。结果表明，平均而言，藻类对鱼类饲料的贡献率为58.5%(LOR)到75.6%(UMR)。食物来源的第二高估计贡献来自C3陆生植物(LOR和UMR分别为21.1%和11.5%)。此外，来自11个单独检查的物种的结果一致地表明，藻类对这些营养协会中的大多数鱼类物种都很重要。由于水文地貌复杂程度的不同，不同河流在相对食物来源方面的差异可能解释了来自藻类的氨基酸的相对比例。
A nearly 40-year debate on the origins of carbon supporting animal production in lotic systems has spawned numerous conceptual theories emphasizing the importance of autochthonous carbon, terrestrial carbon, or both (depending on river stage height). Testing theories has been hampered by lack of adequate analytical methods to distinguish in consumer tissue between ultimate autochthonous and allochthonous carbon. Investigators initially relied on assimilation efficiencies of gut contents and later on bulk tissue stable isotope analysis or fatty acid methods. The newest technique in amino acid, compound specific, stable isotope analysis (AA-CSIA), however, enables investigators to link consumers to food sources by tracing essential amino acids from producers to consumers. We used AA-CSIA to evaluate nutrient sources for 5 invertivorous and 6 piscivorous species in 2 hydrogeomorphically contrasting large rivers: the anastomosing Upper Mississippi River (UMR) and the mostly constricted lower Ohio River (LOR). Museum specimens we analyzed isotopically had been collected by other investigators over many decades (UMR: 1900–1969; LOR: 1931–1970). Our results demonstrate that on average algae contributed 58.5% (LOR) to 75.6% (UMR) of fish diets. The next highest estimated contributions of food sources were from C3 terrestrial plants (21.1 and 11.5% for the LOR and UMR, respectively). Moreover, results from 11 individually examined species consistently demonstrated the importance of algae for most fish species in these trophic guilds. Differences among rivers in relative food source availability resulting from contrasting hydrogeomorphic complexity may account for relative proportions of amino acids derived from algae.
影响因子：
3.7
作者：
Fernandes R;Millard AR;Brabec M;Nadeau MJ;Grootes P
通讯作者：
Grootes P
影响因子：
2.8
作者：
Fernandes, Ricardo;Grootes, Pieter;Nehlich, Olaf
通讯作者：
Nehlich, Olaf