Accurate dispensing of volatile reagents on demand for chemical reactions in EWOD chips.
Accurate dispensing of volatile reagents on demand for chemical reactions in EWOD chips.
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DOI：
10.1039/c2lc40244k
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发表时间：
2012-09-21
期刊：
影响因子：
6.1
通讯作者：
van Dam RM
中科院分区：
文献类型：
--
作者：
Ding H;Sadeghi S;Shah GJ;Chen S;Keng PY;Kim CJ;van Dam RM
数字微流控芯片提供了一个新的平台，用于在微尺度上操纵化学品进行多步化学合成或分析。这些应用所需的有机溶剂和试剂通常是挥发性的，对污染和润湿敏感，即即使在高度疏水的表面上也具有< 90°的接触角（例如，Teflon®或Cytop®），通常用于数字微流控芯片。此外，通常应用规定在气体环境中执行工艺，不允许使用填充液体（例如，油）。这些特性对向芯片输送受控体积的液体提出了挑战。本文介绍了一种自动、简单、准确和可靠的方法，用于从密封的芯片外储液器中输送试剂。该平台通过结合注射泵、简单的片上液体检测器和稳健的接口设计，克服了挥发性溶剂的蒸发损失、交叉污染和芯片溢流的问题。基于阻抗的液体检测仅需要最小的附加硬件来提供反馈信号，以确保将准确体积的挥发性溶剂引入芯片，而不受分配操作之间的时间延迟的影响。乙腈是一种经常使用但由于其润湿性和挥发性而难以处理的溶剂，其多个液滴的按需分配被证明并用于可靠地合成正电子发射断层扫描（PET）探针[18 F]FDG。
Digital microfluidic chips provide a new platform for manipulating chemicals for multi-step chemical synthesis or assays at the microscale. The organic solvents and reagents needed for these applications are often volatile, sensitive to contamination, and wetting, i.e. have contact angles of < 90° even on the highly hydrophobic surfaces (e.g., Teflon® or Cytop®) typically used on digital microfluidic chips. Furthermore, often the applications dictate that the processes are performed in a gas environment, not allowing the use of a filler liquid (e.g., oil). These properties pose challenges for delivering controlled volumes of liquid to the chip. An automated, simple, accurate and reliable method of delivering reagents from sealed, off-chip reservoirs is presented here. This platform overcomes the issues of evaporative losses of volatile solvents, cross-contamination, and flooding of the chip by combining a syringe pump, a simple on-chip liquid detector and a robust interface design. The impedance-based liquid detection requires only minimal added hardware to provide a feedback signal to ensure accurate volumes of volatile solvents are introduced to the chip, independent of time delays between dispensing operations. On-demand dispensing of multiple droplets of acetonitrile, a frequently used but difficult to handle solvent due to its wetting properties and volatility, was demonstrated and used to synthesize the positron emission tomography (PET) probe [18F]FDG reliably.
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影响因子：
6.1
作者：
Gong, Jian;Kim, Chang-Jin (CJ)
通讯作者：
Kim, Chang-Jin (CJ)
DOI：
10.1073/pnas.1117566109
发表时间：
2012-01-17
影响因子：
11.1
作者：
Keng, Pei Yuin;Chen, Supin;van Dama, R. Michael
通讯作者：
van Dama, R. Michael
影响因子：
6.1
作者：
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DOI：
10.1088/0960-1317/18/4/045017
发表时间：
2008-04-01
影响因子：
2.3
作者：
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通讯作者：
Chang, Hwan-You
影响因子：
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作者：
Shih, Steve C. C.;Fobel, Ryan;Wheeler, Aaron R.
通讯作者：
Wheeler, Aaron R.