Characterization of Pitx2c Expression in the Mouse Heart Using a Reporter Transgene
Characterization of Pitx2c Expression in the Mouse Heart Using a Reporter Transgene
复制标题
DOI：
10.1002/dvdy.22492
复制
发表时间：
2011-01-01
影响因子：
2.5
通讯作者：
Harvey, Richard P.
中科院分区：
文献类型：
--
作者：
Furtado, Milena B.;Biben, Christine;Harvey, Richard P.
为了帮助在整个形态发生过程中检测和跟踪心脏左右 (LR) 通路靶向的细胞，详细分析了 Pitx2c-lacZ 转基因 (P2Ztg) 的表达。 P2Ztg 的 β-半乳糖苷酶表达非常强劲，可以可靠地可视化低水平的 Pitx2c 表达，并且几乎完全依赖于心脏中的 NODAL 信号传导。 P2Ztg 在小梁和间隔以及非小梁心肌中表达，并且在与房室管和流出道的个体心内膜垫相关的心肌中存在强烈的表达偏向，这对于心脏间隔至关重要。流出道腹侧表面的表达进化为特定的条纹，可用于在流出道螺旋和重塑过程中追踪未来的主动脉。我们的数据表明，P2Ztg 转基因是检测 LR 级联中分子干扰以及与其他心脏先天性疾病相关的形态发生缺陷的有用资源。发展动态 240: 195-203, 2011。(C) 2010 Wiley-Liss, Inc.
To aid in detection and tracking of cells targeted by the left-right (LR) pathway in the heart throughout morphogenesis, expression from a Pitx2c-lacZ transgene (P2Ztg) was analysed in detail. beta-galactosidase expression from P2Ztg was robust, allowing reliable visualisation of low-level Pitx2c expression, and was virtually entirely dependent upon NODAL signalling in the heart. P2Ztg showed expression in trabecular and septal, as well as non-trabecular, myocardium, and a strong expression bias in myocardium associated with individual endocardial cushions of the atrioventricular canal and outflow tract, which are essential for cardiac septation. Expression on the ventral surface of the outflow tract evolved to a specific stripe that could be used to track the future aorta during outflow tract spiralling and remodelling. Our data show that the P2Ztg transgene is a useful resource for detection of molecular disturbances in the LR cascade, as well as morphogenetic defects associated with other cardiac congenital disorders. Developmental Dynamics 240: 195-203, 2011. (C) 2010 Wiley-Liss, Inc.