Characterization of Ebinur Lake Virus and Its Human Seroprevalence at the China-Kazakhstan Border
Characterization of Ebinur Lake Virus and Its Human Seroprevalence at the China-Kazakhstan Border
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DOI：
10.3389/fmicb.2019.03111
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发表时间：
2020-01-30
影响因子：
5.2
通讯作者：
Yuan, Zhiming
中科院分区：
文献类型：
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作者：
Xia, Han;Liu, Ran;Yuan, Zhiming
近年来，中哈边境贸易和人员往来迅速增加，增加了病媒及其相关疾病传入和输出的潜在风险。艾比湖自然保护区位于新疆维吾尔自治区，靠近中哈边境，生态系统适合蚊子繁殖。在我们以前的工作中，在保护区中分离到一种新的正布尼亚病毒，命名为艾比湖病毒（EBIV）。为了深入了解该地区EBIV的潜在风险，我们进行了一项研究，旨在清楚地概述该地区EBIV的生物学特性及其人类血清阳性率。从系统发育学角度来看，对EBIV所有三个片段的分析表明，它属于正布尼亚病毒属，该病毒聚集在布尼亚维拉血清群中。EBIV在脊椎动物细胞中有效复制并引起细胞病变效应（CPE）。当接种病毒浓度>= 10（4）或10（2）空斑形成单位时，EBIV攻击小鼠的存活率分别为0和20%。感染EBIV的小鼠出现内出血和病理改变。此外，在研究的居民中，总体免疫球蛋白M（IgM）抗体[免疫荧光试验（IFA）1：4]、免疫球蛋白G（IgG）抗体（IFA 1：10）和中和抗体[90%空斑减少中和试验（PRNT）]的患病率分别为8.05%、12.3%和0.95%。总之，EBIV是Bunyamwera血清群的新成员，能够胜任地感染来自蚊子、啮齿动物、猴子或人类的细胞。此外，EBIV在昆明小鼠中引起严重的疾病甚至死亡，并且在当地居民中检测到抗EBIV的抗体，表明该病毒是一种潜在的动物或人类病原体。
In recent years, rapidly increasing trade and travel across the China-Kazakhstan border has increased the potential risk of the introduction and exportation of vectors and their related diseases. The Ebinur Lake Nature Reserve is located in Xinjiang Uygur Autonomous Region, near the China-Kazakhstan border, with a suitable ecosystem for mosquito breeding. In our previous work, a novel Orthobunyavirus species named Ebinur Lake virus (EBIV) was isolated in the reserve. To gain insights into the potential risk of EBIV in this region, we conducted a study that aimed to clearly outline EBIV's biological characteristics and its human seroprevalence in this region. Phylogenetically, the analysis of all three segments of EBIV demonstrated that it belongs to the genus Orthobunyavirus, which is clustered in the Bunyamwera serogroup. EBIV replicated efficiently and caused cytopathic effects (CPEs) in vertebrate cells. The survival rates of the EBIV-challenged mice were 0 and 20% when inoculated with viral concentrations >= 10(4) or 10(2) plaque-forming units, respectively. For EBIV-infected mice, internal bleeding and pathological changes were observed. In addition, the overall immunoglobulin M (IgM) antibody [1:4 by immunofluorescence assay (IFA)], immunoglobulin G (IgG) antibody (1:10 by IFA), and neutralizing antibody [90% plaque reduction neutralization test (PRNT)] prevalence was 8.05, 12.3, and 0.95%, respectively, in the studied residents. In summary, EBIV is a new member of the Bunyamwera serogroup and is able to competently infect cells derived from mosquitoes, rodents, monkeys, or humans. Furthermore, EBIV caused severe disease and even death in challenged Kunming mice, and the antibodies against EBIV have been detected in local residents, indicating that the virus is a potential animal or human pathogen.