Finding fluorescent needles in the cardiac haystack: Tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo three-dimensional fluorescence analysis
Finding fluorescent needles in the cardiac haystack: Tracking human mesenchymal stem cells labeled with quantum dots for quantitative in vivo three-dimensional fluorescence analysis
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DOI：
10.1634/stemcells.2006-0722
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发表时间：
2007-08-01
期刊：
影响因子：
5.2
通讯作者：
Cohen, Ira S.
中科院分区：
文献类型：
--
作者：
Rosen, Amy B.;Kelly, Damon J.;Cohen, Ira S.
干细胞有望修复受损的心脏组织。然而，很少有人确切地知道这些细胞一旦引入体内后的分布。先前追踪递送的干细胞的尝试受到宿主组织的自体荧光和现有标记技术的限制的阻碍。我们已经开发了一种新的负载方法来稳定地标记人骨髓间充质干细胞与量子点（QD）纳米粒子。我们报告了这种长期跟踪技术的优化和验证，并通过将标记细胞输送到哺乳动物心脏来突出几个重要的生物应用。明亮的QD晶体照亮组织切片中的外源性干细胞至少8周后交付，并允许，第一次，在注射到心脏后的所有干细胞的位置的完整的三维重建。
Stem cells show promise for repair of damaged cardiac tissue. Little is known with certainty, however, about the distribution of these cells once introduced in vivo. Previous attempts at tracking delivered stem cells have been hampered by the autofluorescence of host tissue and limitations of existing labeling techniques. We have developed a novel loading approach to stably label human mesenchymal stem cells with quantum dot (QD) nanoparticles. We report the optimization and validation of this long-term tracking technique and highlight several important biological applications by delivering labeled cells to the mammalian heart. The bright QD crystals illuminate exogenous stem cells in histologic sections for at least 8 weeks following delivery and permit, for the first time, the complete three-dimensional reconstruction of the locations of all stem cells following injection into the heart.