Characterisation of GST genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defence
Characterisation of GST genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defence
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DOI：
10.1002/ps.4059
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发表时间：
2016-04-01
影响因子：
4.1
通讯作者：
Chen, Hui
中科院分区：
文献类型：
--
作者：
Dai, Lulu;Ma, Junning;Chen, Hui
树皮甲虫依靠它们的解毒酶来抵抗宿主树木的防御性萜类化合物。谷胱甘肽S-转移酶（GSTs）与细胞色素P450或其他酶协同作用，对有毒化合物进行解毒、螯合或排泄。我们在白松松大小蠹（Dendroctonus armandi）中鉴定了9个新的GST基因，并对推导的全长氨基酸序列进行了生物信息学分析。这些基因属于四个不同的类别（β，sigma，omega和theta）。观察到不同性别之间GST基因转录水平的差异，并且在这些水平内，在以华山松韧皮部喂养并暴露于六种刺激物[（）-（+/-）-柠檬烯和姜黄素]。结论GST基因转录水平的增加可能与寄主植物释放的萜类化合物的解毒作用有关。介导由单萜引起的氧化应激可能是小蠹GST的主要作用。(c)2015年化学工业协会
BACKGROUNDBark beetles rely on their detoxifying enzymes to resist the defensive terpenoids of host trees. Glutathione S-transferases (GSTs) conjugate xenobiotic compounds with a glutathione moiety (GSH) and often work in tandem with cytochromes P450 or other enzymes that aid in the detoxification, sequestration or excretion of toxic compounds.RESULTWe identified nine new GST genes in the Chinese white pine beetle (Dendroctonus armandi) and carried out a bioinformatics analysis on the deduced full-length amino acid sequences. These genes belong to four different classes (epsilon, sigma, omega and theta). Differential transcript levels of each class of GST genes were observed between sexes, and, within these levels, significant differences were found among the different adult substages that were fed phloem of Pinus armandi and exposed to six stimuli [()--pinene, (-)--pinene, (-)--pinene, (+)-3-carene, (+/-)-limonene and turpentine] at 8 and 24 h.CONCLUSION The increased transcription levels of GST genes suggested that they have some relationship with the detoxification of terpenoids that are released by host trees. The mediating oxidative stress that is caused by monoterpene might be the main role of the bark beetle GSTs. (c) 2015 Society of Chemical Industry