Comparison of murine nasal-associated lymphoid tissue and Peyer's patches
Comparison of murine nasal-associated lymphoid tissue and Peyer's patches
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DOI：
10.1164/ajrccm.156.4.97-03017
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发表时间：
1997-10-01
影响因子：
24.7
通讯作者：
McDermott, MR
中科院分区：
文献类型：
--
作者：
Heritage, PL;Underdown, BJ;McDermott, MR
鼻黏膜是第一批接触吸入性抗原的母体。然而，局部免疫反应的性质以及鼻腔相关淋巴组织(NALT)在这些反应中的作用很少被研究。对正常小鼠和霍乱毒素(CT)灌胃或鼻腔免疫的小鼠的NALT和PP细胞进行分离和分析，以确定参与粘膜免疫反应的细胞。与PP细胞相比，未经刺激的NALT细胞含有更高比例的T细胞。NALT细胞制剂中的CD_4/CD_8比值低于PP中的CD_4/CD_8比值，更接近于脾中的CD_4/CD_8比值。此外，NALT细胞分离株的总B细胞频率比PP细胞制剂中观察到的B细胞频率低20%。虽然NALT和PP细胞分离株中既有成熟的B细胞，也有活化表达表面IgA的细胞，但与PP不同的是，NALT没有发现明显的IgA转换细胞，但经CT鼻腔免疫后，在NALT细胞中检测到毒素特异性的IgA抗体形成细胞(AFC)。这些细胞的数量与鼻腔中CT特异性IgA相关，但与肠道洗涤或血清中无关，因此表明鼻腔局部产生抗原特异性粘膜抗体。灌胃CT后，NALT中未检测到抗CTAFCs，鼻腔冲洗液中未检测到CT特异性抗体。这些结果支持这样的观点，即鼻黏膜抗体的产生最好是通过直接刺激IgA承诺的NALT来源的B细胞实现。
The nasal mucosal is the first sire of contact with inhaled antigens. However, the nature of local immune responses and the role of nasal-associated lymphoid tissue (NALT) in those responses have rarely been studied. To characterize the cells involved in mucosally derived immune responses, NALT and Peyer's patch (PP) cells from normal mice, and mice immunized intragastrically or intranasally with cholera toxin (CT), were isolated and analyzed. Compared with PP cells, unstimulated NALT cells contained a higher proportion of T-cells. The CD4:CD8 ratio in NALT cell preparations was less than that observed in PP and more closely resembled that seen in spleen. Additionally, the total B-cell frequency in NALT cell isolates was 20% lower than that observed in PP cell preparations. Although NALT and PP cell isolates contained both mature B-cells and cells undergoing activation to express surface IgA, unlike PP, NALT showed no significant frequency of IgA-switched cells, After intranasal immunization with CT, toxin-specific IgA antibody-forming cells (AFCs) were detected in NALT cell preparations. The numbers of these cells correlated with CT-specific IgA in nasal, but not in gut washes or sera, thus suggesting local nasal production of antigen-specific mucosal antibodies. There was no evidence of anti-CT AFCs in NALT or CT-specific antibody in nasal washes after intragastric CT administration. These results support the notion that nasal mucosal antibody production is best achieved via direct stimulation of IgA-committed, NALT-derived B-cells.